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Anti-HK2 / Hexokinase 2 Antibody (aa74-123) IHC-plus™ LS-B3571

Note: This antibody replaces LS-C81839

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HK2 / Hexokinase 2 Antibodies

Anti-HK2 / Hexokinase 2 Antibody LS-C36657
Rabbit Polyclonal (IgG) to Human HK2 / Hexokinase 2
Human, Monkey
Western blot, Immunoprecipitation, ELISA
Unconjugated
Anti-HK2 / Hexokinase 2 Antibody (aa74-123) IHC-plus™ LS-B3571
Rabbit Polyclonal (IgG) to Human HK2 / Hexokinase 2
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HK2 / Hexokinase 2 Antibody (clone 4C5) LS-C114928
Mouse Monoclonal [clone 4C5] (IgG1) to Human HK2 / Hexokinase 2
Human, Dog
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunofluorescence Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human HK2 / Hexokinase 2
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human HK2 / Hexokinase 2
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

HK2 / Hexokinase 2 antibody was raised against synthetic peptide located between aa74-123 of human HK2 (P52789, NP_000180). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%); Sablefish, Stickleback (92%); Orangutan, Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Zebrafish, Sea squirt (85%).
LS-E19535 - Lyophilized - 100 µg - $145.00
aa74-123
Human HK2 / Hexokinase 2
Immunohistochemistry: LS-B3571 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3571 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About HK2 / Hexokinase 2

P52789 NM_000189 NP_000180.2

HK2 Antibody, Hexokinase II Antibody, Hexokinase type II Antibody, Hexokinase-2, muscle Antibody, HK II Antibody, HXK2 Antibody, Hexokinase-2 Antibody, Muscle form hexokinase Antibody, Hexokinase 2 Antibody, HKII Antibody, Hxk 2 Antibody

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Immunohistochemistry

Immunohistochemistry
Anti-HK2 / Hexokinase 2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3571 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/10/2016

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