Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Rabbit Polyclonal to Rat HK1 / Hexokinase 1
IHC - Frozen, Western blot, Immunoprecipitation
Rat HK1 / Hexokinase 1
Rat (tested or 100% immunogen sequence identity)
IHC - Frozen
Western blot (1:2000)
Specificity and Use
Rat hexokinase type I
Hexokinase type I. No reactivity with hexokinase types II and III by immunoblotting. Reactivity with type I hexokinase from other mammalian species is expected but has not been confirmed experimentally
Western blot: 1:2000. Recognizes the ~110 kD protein. Suggested dilution buffer is Tris buffered saline containing 1% Tween 20. Immunohistochemistry on frozen tissue sections: 1:100 using a fluorescent secondary antibody. Suggested dilution buffer is PBS
Antiserum. No preservative
Store at -20°C. Aliquot to avoid freeze/thaw cycles. Store undiluted.
HK1 Antibody, Brain form hexokinase Antibody, Hexokinase 1 Antibody, Hexokinase type I Antibody, HK I Antibody, HK1-tb Antibody, HK1-tc Antibody, HKI Antibody, Hexokinase type 1 Antibody, HK1-ta Antibody, HXK1 Antibody, Glycolytic enzyme Antibody, Hexokinase-1 Antibody, Type 1 hexokinase Antibody, Type i hexokinase Antibody
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific.