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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HK1 / Hexokinase 1 Antibody (aa78‑108) LS‑C101276
Hexokinase 1 antibody LS-C101276 is an unconjugated rabbit polyclonal antibody to Hexokinase 1 (HK1) from human and mouse. Validated for IHC and WB.
Catalog
Size
Price
LS-C101276-400
400 µl
Unavailable

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Product Description

Hexokinase 1 antibody LS-C101276 is an unconjugated rabbit polyclonal antibody to Hexokinase 1 (HK1) from human and mouse. Validated for IHC and WB.
About HK1 / Hexokinase 1
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. P19367 NM_000188 NP_000179.2

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Specifications

Target
Human HK1 / Hexokinase 1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9527 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C101276. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa78-108
Specificity
This HK1 (Hexokinase) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-108 amino acids from the N-terminal region of human HK1 (Hexokinase).
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

HK1 Antibody (L93) western blot of MCF-7 cell line lysates (35 ug/lane). The HK1 antibody detected the HK1 protein (arrow).
HK1 Antibody (L93) western blot of MCF-7 cell line lysates (35 ug/lane). The HK1 antibody detected the HK1 protein (arrow).

Western blot

The anti-HK1 antibody is used in Western blot to detect HK1 in mouse skeletal muscle tissue lysate.
The anti-HK1 antibody is used in Western blot to detect HK1 in mouse skeletal muscle tissue lysate.

Western blot

Western blot of HK1 (arrow) using HK1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2) (Origene Technologies).
Western blot of HK1 (arrow) using HK1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2) (Origene Technologies).

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

HK1 Antibody (L93) western blot of MCF-7 cell line lysates (35 ug/lane). The HK1 antibody detected the HK1 protein (arrow).
HK1 Antibody (L93) western blot of MCF-7 cell line lysates (35 ug/lane). The HK1 antibody detected the HK1 protein (arrow).

Western blot

The anti-HK1 antibody is used in Western blot to detect HK1 in mouse skeletal muscle tissue lysate.
The anti-HK1 antibody is used in Western blot to detect HK1 in mouse skeletal muscle tissue lysate.

Western blot

Western blot of HK1 (arrow) using HK1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2) (Origene Technologies).
Western blot of HK1 (arrow) using HK1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2) (Origene Technologies).

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

HK1 Antibody (L93) western blot of MCF-7 cell line lysates (35 ug/lane). The HK1 antibody detected the HK1 protein (arrow).
HK1 Antibody (L93) western blot of MCF-7 cell line lysates (35 ug/lane). The HK1 antibody detected the HK1 protein (arrow).

Western blot

The anti-HK1 antibody is used in Western blot to detect HK1 in mouse skeletal muscle tissue lysate.
The anti-HK1 antibody is used in Western blot to detect HK1 in mouse skeletal muscle tissue lysate.

Western blot

Western blot of HK1 (arrow) using HK1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2) (Origene Technologies).
Western blot of HK1 (arrow) using HK1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2) (Origene Technologies).

Immunohistochemistry

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot

HK1 Antibody (L93) western blot of MCF-7 cell line lysates (35 ug/lane). The HK1 antibody detected the HK1 protein (arrow).
HK1 Antibody (L93) western blot of MCF-7 cell line lysates (35 ug/lane). The HK1 antibody detected the HK1 protein (arrow).

Western blot

The anti-HK1 antibody is used in Western blot to detect HK1 in mouse skeletal muscle tissue lysate.
The anti-HK1 antibody is used in Western blot to detect HK1 in mouse skeletal muscle tissue lysate.

Western blot

Western blot of HK1 (arrow) using HK1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2) (Origene Technologies).
Western blot of HK1 (arrow) using HK1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2) (Origene Technologies).


Requested From: 
Date Requested: 6/17/2018

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