Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
- IHC - Paraffin
- Western blot (1:1000)
- Immunoprecipitation (1:50)
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Histone H4 antibody was raised against synthetic peptide (KLH coupled) derived from the amino-terminal sequence of human histone H4 (11kD).
Recognizes endogenous levels of the human histone H4 protein. Does not cross-react with other histones. Species cross-reactivity: Monkey, mouse and rat. Species sequence homology: Drosophila. Note: Adherence to the attached protocol is extremely important.
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:1000; incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunohistochemistry (Paraffin): 1:25.
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.