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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Histone H4 Antibody (N‑Terminus) LS‑C24405
Histone H4 antibody LS-C24405 is an unconjugated rabbit polyclonal antibody to Histone H4 from human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB.
Antibody
Catalog
Size
Price
LS-C24405-100
100 µl
$530

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Product Description

Histone H4 antibody LS-C24405 is an unconjugated rabbit polyclonal antibody to Histone H4 from human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB.
About Histone H4
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is a structural component of the nucleosome, and is subject to covalent modification, including acetylation and methylation, which may alter expression of genes located on DNA associated with its parent histone octamer.

Specifications

Target
Human Histone H4
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:1000)
  • Immunoprecipitation (1:50)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Histone H4 antibody was raised against synthetic peptide (KLH coupled) derived from the amino-terminal sequence of human histone H4 (11kD).
Epitope
N-Terminus
Specificity
Recognizes endogenous levels of the human histone H4 protein. Does not cross-react with other histones. Species cross-reactivity: Monkey, mouse and rat. Species sequence homology: Drosophila. Note: Adherence to the attached protocol is extremely important.
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:1000; incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunohistochemistry (Paraffin): 1:25.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/19/2018

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