Histone H4 antibody LS-C24405 is an unconjugated rabbit polyclonal antibody to Histone H4 from human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB.
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
- IHC - Paraffin
- Western blot (1:1000)
- Immunoprecipitation (1:50)
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Histone H4 antibody was raised against synthetic peptide (KLH coupled) derived from the amino-terminal sequence of human histone H4 (11kD).
Recognizes endogenous levels of the human histone H4 protein. Does not cross-react with other histones. Species cross-reactivity: Monkey, mouse and rat. Species sequence homology: Drosophila. Note: Adherence to the attached protocol is extremely important.
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 1:1000; incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunohistochemistry (Paraffin): 1:25.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.