LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-Histone H4 Antibody (Mono-Methyl-Lys20, clone 5E10-D8, DY488) LS-C180597

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Histone H4 Antibodies

Anti-Histone H4 Antibody (Di-Methyl-Lys20) IHC-plus™ LS-B5032
Mouse Monoclonal (IgG1) to Human Histone H4
Human, Mouse, Rat, Bovine
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-Histone H4 Antibody (Acetyl-Lys5) IHC-plus™ LS-B6121
Rabbit Polyclonal (IgG) to Human Histone H4
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 5E10-D8] (IgG1,k)
Human, Mouse, Rat, Drosophila, Yeast, C. elegans, Mammal
ICC, Western blot, ELISA, Dot Blot (applications tested for the base form of this product only)
DY488 Conjugated

Details

Histone H4
Mouse
Human, Mouse, Rat, Drosophila, Yeast, C. elegans, Mammal (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [5E10-D8]
DyLight 488. Also available Unconjugated or conjugated with Biotin, DY650, DY550.
Purified IgG
Unmodified

Applications

  • ICC (1:200)
  • Western blot (1:1000)
  • ELISA (1:100 - 1:2000)
  • Dot Blot (1:2000)
  • (applications tested for the base form of this product only)

Specificity and Use

Histone H4 antibody was raised against branched peptide found between amino acids 10-100 of Histone H4.
Mono-Methyl-Lys20
Specific for monomethyl histone H4 at lysine 20 (H4Me1K20).
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

50 mM sodium borate
Store at 4°C in the dark.
For research use only.

About Histone H4

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is a structural component of the nucleosome, and is subject to covalent modification, including acetylation and methylation, which may alter expression of genes located on DNA associated with its parent histone octamer.

Western blot

Western blot
Western Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) - Analysis of Histone H4 meIK20 in HeLa histone lysates. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Dot Blot

Dot Blot
Dot Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) - Dot blot analysis of Histone H4 me1K20 with 50 pmol or 10 pmol of peptides corresponding to amino acids 12-31 of Histone H4 bearing unmodified, monomethylated, dimethylated or trimethylated K20. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation
Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) - Hela extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/5/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number