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Anti-Histone H4 Antibody (Di-Methyl-Lys20) IHC-plus™ LS-B5032

Note: This antibody replaces LS-C24397

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Histone H4 Antibodies

Anti-Histone H4 Antibody (Di-Methyl-Lys20) IHC-plus™ LS-B5032
Mouse Monoclonal (IgG1) to Human Histone H4
Human, Mouse, Rat, Bovine
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-Histone H4 Antibody (Acetyl-Lys5) IHC-plus™ LS-B6121
Rabbit Polyclonal (IgG) to Human Histone H4
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human Histone H4
Human, Mouse, Rat, Bovine
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human Histone H4
Mouse
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.01 - 0.1 µg/ml)
  • Immunoprecipitation

Specificity and Use

Histone H4 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 17-28 (RHR[dimeK] VLRDNIQGC) of human histone H4.
Di-Methyl-Lys20
Recognizes methyl-Histone H4, Mr 10kD. Species cross-reactivity: Human, mouse, rat, and bovine. Broad species Cross-reactivity predicted based on conservation of sequence. Does not cross-react with yeast or Tetrahymena.
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 0.01-0.1 ug/ml detects methyl-histone H4 in HeLa acid extracts. Minimal cross-reactivity with unmethylated recombinant Histone H4 is observed if used at these concentrations. Immunohistochemistry: stains Drosophila polytene chromosomes as reported by an independent laboratory. Immunoprecipitation: Immunoprecipitates methyl-Histone H4 from HeLa cell lysates.

Packaging

PBS. Sourced in Ascites.
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months
For research use only.

About Histone H4

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is a structural component of the nucleosome, and is subject to covalent modification, including acetylation and methylation, which may alter expression of genes located on DNA associated with its parent histone octamer.

Immunohistochemistry

Immunohistochemistry
Anti-Histone H4 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5032 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 12/6/2016

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