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| Catalog Number | Size | Price |
|---|---|---|
| LS-B5032-50 | 50 µg (1 mg/ml) | $665 |
IHC‑plus™ Monoclonal Mouse anti‑Human Histone H4 Antibody (Di‑Methyl‑Lys20, IHC, WB) LS‑B5032
IHC‑plus™ Monoclonal Mouse anti‑Human Histone H4 Antibody (Di‑Methyl‑Lys20, IHC, WB) LS‑B5032
Note: This antibody replaces LS-C24397
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
Histone H4 antibody LS-B5032 is an unconjugated mouse monoclonal antibody to Histone H4 (Di-Methyl-Lys20) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Purification
Protein G purified
Modifications
Unmodified
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 17-28 (RHR[dimeK] VLRDNIQGC) of human histone H4.
Epitope
Di-Methyl-Lys20
Specificity
Recognizes methyl-Histone H4, Mr 10kD. Species cross-reactivity: Human, mouse, rat, and bovine. Broad species Cross-reactivity predicted based on conservation of sequence. Does not cross-react with yeast or Tetrahymena.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.01 - 0.1 µg/ml)
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot: 0.01-0.1 ug/ml detects methyl-histone H4 in HeLa acid extracts. Minimal cross-reactivity with unmethylated recombinant Histone H4 is observed if used at these concentrations. Immunohistochemistry: stains Drosophila polytene chromosomes as reported by an independent laboratory. Immunoprecipitation: Immunoprecipitates methyl-Histone H4 from HeLa cell lysates.
Presentation
PBS
Storage
May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Anti-Histone H4 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Histone H4 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ Histone H4 Antibody (Di-Methyl-Lys20) for IHC, WB/Western, IP LS-B5032 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
IHC, Flow Cytometry, ELISA, Dot Blot
Species:
Human
Applications:
IHC, Western blot, Immunoprecipitation, ELISA
Species:
Human, Monkey, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, Western blot, ELISA
Reactivity:
Human, Mouse, Rat
Range:
Positive/Negative
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
