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Anti-Histone H4 Antibody (Acetyl-Lys8) LS-C24387

Catalog Size Price
LS-C24387-100 100 µl Unavailable

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100% Guaranteed
Rabbit Polyclonal (IgG) to Yeast Histone H4
Yeast, Human
Western blot, Immunoprecipitation


Yeast Histone H4
Yeast, Human (tested or 100% immunogen sequence identity)
IgG Polyclonal


  • Western blot
  • Immunoprecipitation

Specificity and Use

Histone H4 antibody was raised against ovalbumin-conjugated, synthetic peptide (RGKGGAcKGLGKG-C) corresponding to aa3-13 of yeast Histone H4 acetylated on lysine 8, with a C-terminal cysteine added for conjugation purposes.
Recognizes acetyl-Histone H4 (Lys8), Mr 10kD. Does not detect Histone H4 from yeast containing a Histone H4 K8R substitution. Species cross-reactivity: Yeast and human. Broad species cross-reactivity is expected.
Suitable for use in Western Blot, Immunoprecipitation and Chromatin IP. Western Blot Analysis: 1:500-1:2000 dilution of this lot detected acetyl-Histone H4 (Lys8) in acid extracts from sodium butyrate treated HeLa cells. Immunoprecipitation: 5 ul of this lot immunoprecipitated acetyl Histone H4 from 1 mg of wild type whole cell yeast lysates but not from a yeast strain containing a Histone H4 K8R substitution. Chromatin IP: Has been reported by an independent laboratory to immunoprecipitate chromatin from wild type yeast strain but not from a yeast strain containing a Histone H4 K8R substitution.


0.01% Thimerosal.
Short term: +4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.

About Histone H4

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is a structural component of the nucleosome, and is subject to covalent modification, including acetylation and methylation, which may alter expression of genes located on DNA associated with its parent histone octamer.

Requested From: 
Date Requested: 3/23/2018

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