(tested or 100% immunogen sequence identity)
- Western blot
Histone H4 antibody was raised against ovalbumin-conjugated, synthetic peptide (SGRGAcKGGKGL-C) corresponding to aa1-10 of yeast histone H4 acetylated on lysine 5, with a C-terminal cysteine added for conjugation purposes.
Recognizes acetyl-Histone H4 (Lys5). Species cross-reactivity: Yeast and human. Broad species cross-reactivity is expected.
Suitable for use in Western Blot and Chromatin IP. Western Blot Analysis: 1:1000-1:5000 dilution of this lot detected acetyl-Histone H4 (Lys5) in acid extracts from sodium butyrate treated HeLa cells. Chromatin IP: An independent laboratory has shown that this antibody preferentially immunoprecipitates chromatin from wild type yeast, compared to yeast containing a histone H4 K5R substitution.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.