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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Histone H4 Antibody (Acetyl‑Lys12) LS‑C24398
Histone H4 antibody LS-C24398 is an unconjugated rabbit polyclonal antibody to Histone H4 from human and yeast. Validated for Func, IP and WB.
Antibody
Catalog
Size
Price
LS-C24398-100
100 µl
$710

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Product Description

Histone H4 antibody LS-C24398 is an unconjugated rabbit polyclonal antibody to Histone H4 from human and yeast. Validated for Func, IP and WB.
About Histone H4
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is a structural component of the nucleosome, and is subject to covalent modification, including acetylation and methylation, which may alter expression of genes located on DNA associated with its parent histone octamer.

Specifications

Target
Histone H4
Host
Rabbit
Reactivity
Human, Yeast (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • Western blot (1:3000)
  • Immunoprecipitation
  • Functional Assay
Immunogen
Histone H4 antibody was raised against ovalbumin-conjugated, synthetic peptide containing the sequence (. LG[acK]GG.) in which acK corresponds to acetyl-lysine 12 of yeast Histone H4.
Epitope
Acetyl-Lys12
Specificity
Recognizes histone H4, Mr 10kD. Species cross-reactivity: Yeast and human. Broad species cross-reactivity expected.
Usage
Suitable for use in Peptide Inhibition, Peptide Dot Blot, Western Blot and Immunoprecipitation. Peptide Inhibition: 10 uM of a histone H4 peptide containing acetyl-lysine 12 abolished detection of histone H4 by anti-acetyl-Histone H4 (Lys12) in immunoblot analysis of HeLa acid extracts. Little to no signal reduction was observed with 10 uM of histone H4 peptides containing acetyl-lysine 5, acetyl-lysine 8 or acetyl-lysine 16. Peptide Dot Blot: A 1:1000 detects serial dilutions (25, 5, 1 and 0.2uM) of a histone H4 peptide containing acetyl-lysine 12 spotted onto PVDF, and not histone H4 peptides containing acetyl-lysine 5, acetyl-lysine 8 or acetyl-lysine 16. Western Blot: 1:3000 to 1:10000 detects acetyl-Histone H4 (Lys12) in acid extracted proteins from HeLa cells. The antibody did not detect unacetylated recombinant Histone H4. Immunoprecipitation: 2-6 ul immunoprecipitated the Ime2 promoter from wild type yeast chromatin and not from mutant yeast chromatin containing a histone H4 K12R mutation.
Presentation
0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/18/2018

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