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Anti-Histone H4 Antibody (Acetyl-Lys12) LS-C24398

Ordering

Wt. Vol. Conc. Price
- 100 µl - $620
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular Histone H4 Antibodies

Anti-Histone H4 Antibody (Di-Methyl-Lys20) IHC-plus™ LS-B5032
Mouse Monoclonal (IgG1) to Human Histone H4
Human, Mouse, Rat, Bovine
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-Histone H4 Antibody (Acetyl-Lys5) IHC-plus™ LS-B6121
Rabbit Polyclonal (IgG) to Human Histone H4
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG)
Human, Yeast
Western blot, Immunoprecipitation, Functional Assay
Unconjugated

Details

Histone H4
Rabbit
Human, Yeast (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • Western blot (1:3000)
  • Immunoprecipitation
  • Functional Assay

Specificity and Use

Histone H4 antibody was raised against ovalbumin-conjugated, synthetic peptide containing the sequence (. LG[acK]GG.) in which acK corresponds to acetyl-lysine 12 of yeast Histone H4.
Acetyl-Lys12
Recognizes histone H4, Mr 10kD. Species cross-reactivity: Yeast and human. Broad species cross-reactivity expected.
Suitable for use in Peptide Inhibition, Peptide Dot Blot, Western Blot and Immunoprecipitation. Peptide Inhibition: 10 uM of a histone H4 peptide containing acetyl-lysine 12 abolished detection of histone H4 by anti-acetyl-Histone H4 (Lys12) in immunoblot analysis of HeLa acid extracts. Little to no signal reduction was observed with 10 uM of histone H4 peptides containing acetyl-lysine 5, acetyl-lysine 8 or acetyl-lysine 16. Peptide Dot Blot: A 1:1000 detects serial dilutions (25, 5, 1 and 0.2uM) of a histone H4 peptide containing acetyl-lysine 12 spotted onto PVDF, and not histone H4 peptides containing acetyl-lysine 5, acetyl-lysine 8 or acetyl-lysine 16. Western Blot: 1:3000 to 1:10000 detects acetyl-Histone H4 (Lys12) in acid extracted proteins from HeLa cells. The antibody did not detect unacetylated recombinant Histone H4. Immunoprecipitation: 2-6 ul immunoprecipitated the Ime2 promoter from wild type yeast chromatin and not from mutant yeast chromatin containing a histone H4 K12R mutation.

Packaging

0.05% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About Histone H4

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is a structural component of the nucleosome, and is subject to covalent modification, including acetylation and methylation, which may alter expression of genes located on DNA associated with its parent histone octamer.

Requested From: United States
Date Requested: 12/5/2016

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