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Anti-Histone H4 Antibody (aa15-30) IHC-plus™ LS-B2036

Note: This antibody replaces LS-C695, LS-C67994
Catalog Size Price
LS-B2036-50 50 µg (0.5 mg/ml) Unavailable

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100% Guaranteed
Rabbit Polyclonal (IgG) to Human Histone H4
Human, Monkey, Mouse, Rat, Chicken, Xenopus, Zebrafish, Drosophila
IHC - Paraffin, Western blot
Unconjugated

Details

Human Histone H4
Rabbit
Human, Monkey, Mouse, Rat, Chicken, Xenopus, Zebrafish, Drosophila (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (2 µg/ml)

Specificity and Use

Histone H4 antibody was raised against synthetic peptide from human Histone H4.
aa15-30
A 14 kD band is observed. The has been evaluated in human, and most likely reacts with additional mammalian, invertebrate, and plant species as the immunogen sequence used to make it is conserved.
Immunohistochemistry: LS-B2036 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2036 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About Histone H4

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H4 is a structural component of the nucleosome, and is subject to covalent modification, including acetylation and methylation, which may alter expression of genes located on DNA associated with its parent histone octamer.

Immunohistochemistry

Immunohistochemistry
Anti-Histone H4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2036 concentration 5 ug/ml.

Western blot

Western blot
Western blot of Histone H4 in human PBMC cell lysate with antibody.

Requested From: 
Date Requested: 6/25/2017

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