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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Histone H3.3 Antibody (aa1‑86) LS‑C186407
Histone H3.3 antibody LS-C186407 is an unconjugated rabbit polyclonal antibody to human Histone H3.3. Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C186407-100
100 µl (0.44 mg/ml)
Unavailable

Popular Histone H3.3 Products

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Anti-H3F3A antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7958 dilution 10 ug/ml.
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Applications: IHC, IHC - Paraffin, Western blot
Anti-H3F3B antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8442 dilution 15 ug/ml.
Species: Human
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Western blot of Histone H3.3 antibody
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

Histone H3.3 antibody LS-C186407 is an unconjugated rabbit polyclonal antibody to human Histone H3.3. Validated for ICC, IHC and WB.

Specifications

Target
Human Histone H3.3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Histone H3.3 antibody was raised against recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 86 of Histone H3.3B.
Epitope
aa1-86
Specificity
Human H3F3B
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 20% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded HSC3 xenograft using Histone H3.3B antibody at 1:500 dilution.
IHC of paraffin-embedded HSC3 xenograft using Histone H3.3B antibody at 1:500 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using Histone H3.3B antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using Histone H3.3B antibody at 1:200 dilution.

Western blot

Histone H3.3B antibody detects H3F3B protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 15 % SDS-PAGE. Histone H3.3B antibody dilution:1:1000
Histone H3.3B antibody detects H3F3B protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 15 % SDS-PAGE. Histone H3.3B antibody dilution:1:1000

Western blot

Sample (50 ug of whole cell lysate). A: mouse brain. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: mouse brain. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Hela. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hela. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded HSC3 xenograft using Histone H3.3B antibody at 1:500 dilution.
IHC of paraffin-embedded HSC3 xenograft using Histone H3.3B antibody at 1:500 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using Histone H3.3B antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using Histone H3.3B antibody at 1:200 dilution.

Western blot

Histone H3.3B antibody detects H3F3B protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 15 % SDS-PAGE. Histone H3.3B antibody dilution:1:1000
Histone H3.3B antibody detects H3F3B protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 15 % SDS-PAGE. Histone H3.3B antibody dilution:1:1000

Western blot

Sample (50 ug of whole cell lysate). A: mouse brain. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: mouse brain. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Hela. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hela. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded HSC3 xenograft using Histone H3.3B antibody at 1:500 dilution.
IHC of paraffin-embedded HSC3 xenograft using Histone H3.3B antibody at 1:500 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using Histone H3.3B antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using Histone H3.3B antibody at 1:200 dilution.

Western blot

Histone H3.3B antibody detects H3F3B protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 15 % SDS-PAGE. Histone H3.3B antibody dilution:1:1000
Histone H3.3B antibody detects H3F3B protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 15 % SDS-PAGE. Histone H3.3B antibody dilution:1:1000

Western blot

Sample (50 ug of whole cell lysate). A: mouse brain. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: mouse brain. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Hela. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hela. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.

Immunohistochemistry

IHC of paraffin-embedded HSC3 xenograft using Histone H3.3B antibody at 1:500 dilution.
IHC of paraffin-embedded HSC3 xenograft using Histone H3.3B antibody at 1:500 dilution.

Immunocytochemistry

Immunofluorescence of paraformaldehyde-fixed HeLa using Histone H3.3B antibody at 1:200 dilution.
Immunofluorescence of paraformaldehyde-fixed HeLa using Histone H3.3B antibody at 1:200 dilution.

Western blot

Histone H3.3B antibody detects H3F3B protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 15 % SDS-PAGE. Histone H3.3B antibody dilution:1:1000
Histone H3.3B antibody detects H3F3B protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 15 % SDS-PAGE. Histone H3.3B antibody dilution:1:1000

Western blot

Sample (50 ug of whole cell lysate). A: mouse brain. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.
Sample (50 ug of whole cell lysate). A: mouse brain. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.

Western blot

Sample (30 ug of whole cell lysate). A: Hela. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: Hela. 15% SDS PAGE. H3F3B antibody diluted at 1:1000.


Requested From: 
Date Requested: 6/22/2018

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