(tested or 100% immunogen sequence identity)
- IHC (1:5000)
- ICC (1:5000)
- Western blot (1:1000 - 1:10000)
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Histone H2B antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 6-16 of human histone H2B (C-SAPAPKKG[pS]KK).
Recognizes phospho-Histone H2B (Ser14), Mr 15kD. Specific for the phosphorylated peptide and does not cross-react with the unphosphorylated counterpart. Species cross-reactivity: Human. Broad species cross-reactivity expected based on sequence homology.
Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: 1:1000-1:10000. Detected phospho-Histone H2B in HL-60 cells treated with 20 ug/ul VP16 for 18 hours. A 1:250-1:2000 dilution also detected phospho-Histone H2B acid extracted proteins from colcemid-arrested HeLa cells. Immunocytochemistry: 1:5000 selectively immunostained pycnotic apoptotic HL-60 cells and TUNEL positive HeLa cells. Immunohistochemistry: 1:5000 immunostained deparaffinized Xenopus tail sections. Note: Use of this antibody at dilutions which are too low will result in non-specific detection of Histone H2B and other unknown proteins in immunoblots of acid extracts from untreated cells.
0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.