(tested or 100% immunogen sequence identity)
Histone H2B antibody was raised against kLH-conjugated, synthetic peptide containing. PS[AcK] SA. in which AcK corresponds acetyl-lysine at residue 5 of human Histone H2B.
Recognizes acetylated Histone H2B, Mr ~14-16kD. Species cross-reactivity: Human. Predicted to cross-react with mouse based sequence conservation.
Suitable for use in Western Blot. Western Blot Analysis: 1:500-1:2000 dilution of this lot detected acetylated Histone H2B in sodium butyrate treated, acid extracted proteins from HeLa cells. Unacetylated recombinant Histone H2B was not detected.
0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.