Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin
- Western blot (1:1000)
- Immunoprecipitation (1:50)
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Histone H2B antibody was raised against synthetic acetyl-Lys5 peptide (KLH coupled) corresponding to residues surrounding Lys5 of human histone H2b.
Acetyl-Histone H2B (Lys5) Antibody detects endogenous levels of Lys5 acetylated histone H2B. The antibody does not cross-react with other acetylated histones. Species cross-reactivity: Monkey, mouse and rat. Note: Adherence to the attached protocol is extremely important.
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry (Paraffin sections) and Immunocytochemistry (ABC detection method). Western Blot: 1:1000. Immunoprecipitation: 1:50. Immunohistochemistry (Paraffin): 1:100. Immunocytochemistry (ABC): 1:50.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
For research use only.
About Histone H2B
Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the 'beads on a string' structure.