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| Catalog Number | Size | Price |
|---|---|---|
| LS-C24282-200 | 200 µg | $839 |
Polyclonal Rabbit anti‑Human Histone H2B Antibody (aa118‑126, WB) LS‑C24282
Polyclonal Rabbit anti‑Human Histone H2B Antibody (aa118‑126, WB) LS‑C24282
Antibody:
Histone H2B Rabbit anti-Human Polyclonal (aa118-126) Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Histone H2B Rabbit anti-Human Polyclonal (aa118-126) Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
Histone H2B antibody LS-C24282 is an unconjugated rabbit polyclonal antibody to human Histone H2B (aa118-126). Validated for WB.
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 118-126 (CGAVTKYTSSK) of human Histone H2B, with an N-Terminus CG added for conjugation purposes. Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (90%).
Epitope
aa118-126
Specificity
Recognizes and is specific for Histone H2B, Mr ~15kD. An additional band at 23kD is detected in some samples, which likely corresponds to ubiquityl-Histone H2B. Species cross-reactivity: Human, chicken and Xenopus. Others not tested. Broad species cross-reactivity expected based on sequence homology.
Applications
- Western blot (1:1000 - 1:10000)
Usage
Suitable for use in Western Blot. Western Blot: 1:1000-1:10000 detected Histone H2B in acid extracts from either sodium butyrate or colcemid treated HeLa cells and 13-114 respectively), chicken core histone and recombinant Xenopus Histone H2B.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat, Bovine, Chicken, Zebrafish, Drosophila, Insect
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat, Chicken
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Bovine
Applications:
Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat, Bovine
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
