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Anti-Histone H2B Antibody (aa118-126) LS-C24282

Catalog Size Price
LS-C24282-200 200 µg Unavailable

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100% Guaranteed
Rabbit Polyclonal (IgG) to Human Histone H2B
Western blot (applications tested for the base form of this product only)


Human Histone H2B
Human (tested or 100% immunogen sequence identity)
Mouse, Rat, Bovine, Chicken, Zebrafish, Drosophila, Insect (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Protein A purified


  • Western blot (1:1000 - 1:10000)
  • (applications tested for the base form of this product only)

Specificity and Use

Histone H2B antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 118-126 (CGAVTKYTSSK) of human Histone H2B, with an N-terminal CG added for conjugation purposes. Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (90%).
Recognizes and is specific for Histone H2B, Mr ~15kD. An additional band at 23kD is detected in some samples, which likely corresponds to ubiquityl-Histone H2B. Species cross-reactivity: Human, chicken and Xenopus. Others not tested. Broad species cross-reactivity expected based on sequence homology.
Suitable for use in Western Blot. Western Blot: 1:1000-1:10000 detected Histone H2B in acid extracts from either sodium butyrate or colcemid treated HeLa cells and 13-114 respectively), chicken core histone and recombinant Xenopus Histone H2B.


200 ul of 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide.
Short term: +4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.

About Histone H2B

Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the 'beads on a string' structure.

Requested From: 
Date Requested: 3/19/2018

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