(tested or 100% immunogen sequence identity)
Histone H2A antibody was raised against kLH-conjugated, synthetic peptide (GKQGG[acK]ARAKAK-C) corresponding to aa4-15 of human Histone H2A, to which a C-terminal cysteine was added for conjugation purposes. The immunizing sequence has identical aa in chicken, bovine, rat, mouse and duck.
Recognizes acetylated Histone 2A, Mr 14kD. Species cross-reactivity: Human. Broad species cross-reactivity expected due to conserved sequence of the immunogen.
Suitable for use in Western Blot. Western Blot Analysis: 1:500-1:20000 dilution of this lot detected acetylated Histone H2A in sodium butyrate treated acid extracted proteins from HeLa cell.
0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.