Histone H2A antibody LS-C24270 is an unconjugated rabbit polyclonal antibody to Histone H2A from human, mouse and rat. Validated for WB.
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Histone H2A antibody was raised against kLH-conjugated, synthetic peptide containing (. GRG[AcK]QGG[AcK]ARA[AcK]A[AcK]TRS.), in which AcK corresponds to acetyl-lysine residues 5, 9, 13 and 15 of human Histone H2A.
Recognizes acetylated Histone H2A, Mr ~14kD. Species cross-reactivity: Human. Predicted to cross-react with mouse and rat based on sequence conservation.
Suitable for use in Western Blot. Western Blot Analysis: 1:500-1:10000 dilution of this lot detected acetylated Histone H2A in acid extracted proteins from sodium butyrate treated HeLa cells. Unacetylated recombinant Histone H2A was not detected.
0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.