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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Histone H2A Antibody (Acetyl‑Lys5) LS‑C24263
Antibody
Histone H2A antibody LS-C24263 is an unconjugated rabbit polyclonal antibody to Histone H2A from human, mouse, rat and other species. Validated for ELISA, ICC, IHC, IP and WB.
Catalog
Size
Price
LS-C24263-100
100 µl (0.005 mg/ml)
Unavailable

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Product Description

Histone H2A antibody LS-C24263 is an unconjugated rabbit polyclonal antibody to Histone H2A from human, mouse, rat and other species. Validated for ELISA, ICC, IHC, IP and WB.
About Histone H2A
Histone H2A is composed of non-allelic variants. The term "Histone H2A" is intentionally non-specific and refers to a variety of closely related proteins that vary often by only a few amino acids. Notable variants include H2A.1, H2A.2, H2A.X, and H2A.Z. Changes in variant composition occur in differentiating cells. This was observed in differentiating neurons during synthesis and turnover; changes in variant composition were seen among the H2A.1 histone.

Specifications

Target
Human Histone H2A
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot (1:1000)
  • Immunoprecipitation (1:50)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Histone H2A antibody was raised against synthetic acetyl-peptide KLH coupled) corresponding to residues surrounding Lys5 of human histone H2A.
Epitope
Acetyl-Lys5
Specificity
Recognizes human Acetyl-Histone H2A (Lys5) Antibody detects histone H2A only when acetylated at Lys5. This antibody does not cross-react with other acetylated histones. Species cross-reactivity: Monkey, mouse and rat.
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunohistochemistry (Paraffin): 1:50. Immunocytochemistry (ABC): 1:100.
Presentation
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/20/2018

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