Histone H2A antibody LS-C24263 is an unconjugated rabbit polyclonal antibody to Histone H2A from human, mouse, rat and other species. Validated for ELISA, ICC, IHC, IP and WB.
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin
- Western blot (1:1000)
- Immunoprecipitation (1:50)
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Histone H2A antibody was raised against synthetic acetyl-peptide KLH coupled) corresponding to residues surrounding Lys5 of human histone H2A.
Recognizes human Acetyl-Histone H2A (Lys5) Antibody detects histone H2A only when acetylated at Lys5. This antibody does not cross-react with other acetylated histones. Species cross-reactivity: Monkey, mouse and rat.
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunohistochemistry (Paraffin): 1:50. Immunocytochemistry (ABC): 1:100.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.