Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Protein A purified
- IHC - Paraffin
- Western blot (1:1000)
- Immunoprecipitation (1:50)
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Histone H2A antibody was raised against synthetic acetyl-peptide KLH coupled) corresponding to residues surrounding Lys5 of human histone H2A.
Recognizes human Acetyl-Histone H2A (Lys5) Antibody detects histone H2A only when acetylated at Lys5. This antibody does not cross-react with other acetylated histones. Species cross-reactivity: Monkey, mouse and rat.
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunohistochemistry (Paraffin): 1:50. Immunocytochemistry (ABC): 1:100.
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.