(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- ICC (5 - 10 µg/ml)
- Western blot (1 - 2 µg/ml)
- (applications tested for the base form of this product only)
Histone H1 antibody was raised against hyper-phosphorylated isoform of Tetrahymena Histone H1.
Recognizes phosphorylated histone H1 (32kD). Additional proteins (Mr 66 and 100kD) were detected. Species cross-reactivity: human, bovine.
Suitable for use in Western Blot and Immunocytochemistry. Proteins were visualized using a goat anti-rabbit secondary antibody labeled with HRP. A chemiluminescence detection system was used. Immunocytochemistry: 5-10 ug/ml showed positive immunostaining for Histone H1 in mitotic HeLa cells fixed with 95% ethanol, 5% glacial acetic acid.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.