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Anti-Histone H1 Antibody (Hyperphosphorylated) LS-C24260

Ordering

Wt. Vol. Conc. Price
200 µg - - $620
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human Histone H1
Human
ICC, Western blot
Unconjugated

Details

Human Histone H1
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • ICC (5 - 10 µg/ml)
  • Western blot (1 - 2 µg/ml)

Specificity and Use

Histone H1 antibody was raised against hyper-phosphorylated isoform of Tetrahymena Histone H1.
Hyperphosphorylated
Recognizes phosphorylated histone H1 (32kD). Additional proteins (Mr 66 and 100kD) were detected. Species cross-reactivity: human, bovine.
Suitable for use in Western Blot and Immunocytochemistry. Proteins were visualized using a goat anti-rabbit secondary antibody labeled with HRP. A chemiluminescence detection system was used. Immunocytochemistry: 5-10 ug/ml showed positive immunostaining for Histone H1 in mitotic HeLa cells fixed with 95% ethanol, 5% glacial acetic acid.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About Histone H1

Histone H1 is one of the five main histone protein families which are components of chromatin in eukaryotic cells. Though highly conserved, it is nevertheless the most variable histone in sequence across species. Metazoan H1 proteins feature a central globular domain and long C- and short N-terminal tails. H1 is involved with the packing of the "beads on a string" sub-structures into a high order structure, whose details have not yet been solved.

Requested From: United States
Date Requested: 12/9/2016

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