Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Specificity and Use
Histone H1/H5 antibody was raised against native histone H5 protein purified from chicken erythrocytes. Epitope recognized by this antibody was mapped to amino acid residues 53-98.
Recognizes chicken Histones H1? and H5, Mr 30kD. Species cross-reactivity: Human, mouse, rat and bovine.
Suitable for use in Western Blot and Immunocytochemistry. Western Blot Analysis: 1-2 ug/ml detects Histones H1o and H5 in HeLa acid precipitated lysate. Hela acid precipitated lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Histone H1o/H5 (2 ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: Reported to detect positive immunostaining for Histone H1? and H5 in KD human fibroblasts cells fixed and permeabilized with methanol and acetone at -20?C.