Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Rat (tested or 100% immunogen sequence identity)
IHC - Paraffin
IHC - Frozen
Immunofluorescence (1:300 - 1:600)
Specificity and Use
Histamine antibody was raised against histamine conjugated to BSA.
Recognizes Histamine. Absorption with 10-100 ug histamine per ml diluted antiserum abolishes the staining, while noradrenaline, 5-HT, VIP, glucagon and histidine do not. Species cross-reactivity: Human and rat.
Suitable for use in Immunohistochemistry and Immunofluorescence. Immunohistochemistry (Frozen and paraffin sections): 1:1500-1:2000 (PAP). Immunofluorescence: 1:300-1:600 with overnight incubation at 4?C. Positive control: Cryostat sections of carbodiimide fixed human skin or freeze-dried paraffin-sections (vapor fixed in diethylpyrocarbonate; DEPC) of rat stomach. Able to recognize free histamine.
50 µl Reconstitute with 50ul sterile ddH2O.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.