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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HIST1H2BN Antibody (aa1‑30) LS‑C166898 Best Seller
HIST1H2BN antibody LS-C166898 is an unconjugated rabbit polyclonal antibody to human HIST1H2BN. Validated for IHC and WB.
Catalog
Size
Price
LS-C166898-400
400 µl
Unavailable

Popular HIST1H2BN Products

Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, Western blot, ELISA
Immunohistochemistry Dilution at 1:20 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA
Immunohistochemistry Dilution at 1:10 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA
Immunohistochemistry Dilution at 1:10 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

HIST1H2BN antibody LS-C166898 is an unconjugated rabbit polyclonal antibody to human HIST1H2BN. Validated for IHC and WB.
About HIST1H2BN
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. Q99877 Q99877

HIST1H2BN Antibody, Histone H2B type 1-N Antibody, Histone 1, H2bn Antibody, Histone cluster 1, H2bn Antibody, Histone H2B.d Antibody


Specifications

Target
Human HIST1H2BN
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E2196 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C166898. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1-30
Specificity
This HIST1H2BN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H2BN.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

HIST1H2BN Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human lung tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
HIST1H2BN Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human lung tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

HIST1H2BN Antibody western blot of CEM cell line lysates (35 ug/lane). The HIST1H2BN antibody detected the HIST1H2BN protein (arrow).
HIST1H2BN Antibody western blot of CEM cell line lysates (35 ug/lane). The HIST1H2BN antibody detected the HIST1H2BN protein (arrow).

Immunohistochemistry

HIST1H2BN Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human lung tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
HIST1H2BN Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human lung tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

HIST1H2BN Antibody western blot of CEM cell line lysates (35 ug/lane). The HIST1H2BN antibody detected the HIST1H2BN protein (arrow).
HIST1H2BN Antibody western blot of CEM cell line lysates (35 ug/lane). The HIST1H2BN antibody detected the HIST1H2BN protein (arrow).

Immunohistochemistry

HIST1H2BN Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human lung tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
HIST1H2BN Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human lung tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

HIST1H2BN Antibody western blot of CEM cell line lysates (35 ug/lane). The HIST1H2BN antibody detected the HIST1H2BN protein (arrow).
HIST1H2BN Antibody western blot of CEM cell line lysates (35 ug/lane). The HIST1H2BN antibody detected the HIST1H2BN protein (arrow).

Immunohistochemistry

HIST1H2BN Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human lung tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
HIST1H2BN Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human lung tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

HIST1H2BN Antibody western blot of CEM cell line lysates (35 ug/lane). The HIST1H2BN antibody detected the HIST1H2BN protein (arrow).
HIST1H2BN Antibody western blot of CEM cell line lysates (35 ug/lane). The HIST1H2BN antibody detected the HIST1H2BN protein (arrow).


Requested From: 
Date Requested: 6/20/2018

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