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His Tag
A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The tag serves as a vector for the induced expression and production of that protein. His-tags are useful for protein purification due to the strong ionic affinity of histidines to various metals used in chromatography columns that allow for a tagged protein to be selected for in high purity.
His Tag Target Details
| Target Name: | His Tag |
Publications (5)
1
Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain. Yi CE, Ba L, Zhang L, Ho DD, Chen Z. Journal of virology. 2005 79:11638-46.
[PubMed:16140741]
[PMC:PMC1212612]
Related Antibodies: LS-C60077.
2
Allosteric gating of Son of sevenless activity by the histone domain. Yadav KK, Bar-Sagi D. Proceedings of the National Academy of Sciences of the United States of America. 2010 107:3436-40.
[PubMed:20133694]
[PMC:PMC2840464]
Related Antibodies: LS-C157807.
3
The Staphylococcus aureus superantigen SElX is a bifunctional toxin that inhibits neutrophil function. Tuffs SW, James DBA, Bestebroer J, Richards AC, Goncheva MI, O'Shea M, Wee BA, Seo KS, Schlievert PM, Lengeling A, van Strijp JA, Torres VJ, Fitzgerald JR. PLoS pathogens. 2017 13:e1006461. (Flo; Human)
[Full Text Article]
[PubMed:28880920]
[PMC:PMC5589267]
Related Antibodies: LS-C57341.
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(158)
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His Tag
(159)
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(156)
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(3)
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(1)
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His Tag Rabbit Polyclonal Antibody
ELISA, IHC, WB
Unconjugated
100 µg/$420
His Tag Mouse anti-Synthetic Monoclonal (FITC) (AD1.1.10) Antibody
Synthetic
Flo, IF
FITC Conjugated
0.1 mg/$444
His Tag Goat Polyclonal Antibody
ELISA, IF, IHC, WB
Unconjugated
100 µg/$832
His Tag Goat Polyclonal Antibody
ELISA, ICC, IP, WB
Unconjugated
100 µg/$500
His Tag Mouse Monoclonal (HRP) Antibody
ELISA, IHC, WB
HRP Conjugated
100 µl/$801
His Tag Mouse Monoclonal (Biotin) (33D10.D2.G8) Antibody
ELISA, IHC, WB
Biotin Conjugated
100 µg/$552
His Tag Mouse anti-Synthetic Monoclonal (AD1.1.10) Antibody
Synthetic
ELISA, IP, WB
Unconjugated
0.1 mg/$332
![His Tag Antibody - Anti-6X His epitope tag Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com/image2/his-tag-antibody-hrp-ls-c154274/81964_5162530.jpg)
His Tag Rabbit Polyclonal (HRP) Antibody
ELISA, IHC, WB
HRP Conjugated
100 µg/$529
His Tag Mouse Monoclonal (33D10.D2.G8) Antibody
ELISA, IP, WB
Unconjugated
100 µg/$443
His Tag Rabbit Polyclonal (His epitope) Antibody
ELISA, WB
Unconjugated
40 µg/$297
His Tag Mouse Monoclonal (AP) Antibody
ELISA, WB
AP Conjugated
100 µl/$801
His Tag Mouse Monoclonal (HRP) (33D10.D2.G8) Antibody
ELISA, WB
HRP Conjugated
100 µg/$552
![His Tag Antibody - Western blot of His-tagged fusion proteins using THETM His Antibody, mAb, Mouse (THETM His Tag Antibody, mAb, Mouse, 1 ug/ml) Lane 1: N-terminal His-tagged fusion protein Lane 2: C-terminal His-tagged fusion protein The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody and LumiSensor HRP Substrate Kit.](https://lsbio-7d62.kxcdn.com/image2/his-tag-antibody-clone-6g2a9-ls-c51081/127890_5162492.jpg)
His Tag Mouse Monoclonal (6G2A9) Antibody
ELISA, Flo, IF, IP, WB
Unconjugated
100 µg/$538
His Tag Mouse Monoclonal Antibody
WB
Unconjugated
200 µl/$393
His Tag Mouse Monoclonal Antibody
ELISA, IF, IP, WB
Unconjugated
50 µl/$434
Competitive EIA
96-Well Strip Plate
All species
24.69 - 2000 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
1 Plate/$942
His Tag Goat Polyclonal (HRP) Antibody
ELISA, ICC, WB
HRP Conjugated
100 µg/$577
His Tag Mouse Monoclonal Antibody
ELISA, Flo, IF, IP, WB
Unconjugated
100 µg/$832
![His Tag Antibody - Anti-6X His Epitope Tag Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com/image2/his-tag-antibody-biotin-ls-c154291/81972_5162529.jpg)
His Tag Rabbit Polyclonal (Biotin) Antibody
ELISA, IHC, WB
Biotin Conjugated
100 µg/$529
![His Tag Antibody - Western blot of His tagged fusion protein using THETM His Tag Antibody [Biotin], mAb, mouse (THETM His Tag Antibody [Biotin], mAb, Mouse, 1 ug/ml) The signal was developed with Streptavidin-HRP and and LumiSensor HRP Substrate Kit Predicted Size: 52 KD Observed Size: 52 KD This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com/image2/his-tag-antibody-clone-6g2a9-biotin-ls-c51495/128050_281331.jpg)
His Tag Mouse Monoclonal (Biotin) (6G2A9) Antibody
ELISA, WB
Biotin Conjugated
100 µg/$572
His Tag Rabbit Polyclonal Antibody
ELISA, IF, IHC, IP, WB
Unconjugated
100 µg/$343
His Tag Mouse Monoclonal (HIS.H8) Antibody
IF, IP, WB
Unconjugated
100 µg/$316
![His Tag Antibody - Lane 1: Marker Lane 2: 10 ng Multiple Tag Cell Lysate fusion protein Lane 3: 1 ng Multiple Tag Cell Lysate fusion protein Lane 4: Negative control Detect antibody: 1 ug/ml THETM Anti-His [HRP] Monoclonal Antibody THETM His Tag Antibody [HRP], mAb, Mouse This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com/image2/his-tag-antibody-clone-6g2a9-hrp-ls-c51494/128049_281327.jpg)
His Tag Mouse Monoclonal (HRP) (6G2A9) Antibody
ELISA, WB
HRP Conjugated
100 µg/$572
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