- IHC (1:500 - 1:2000)
- Western blot (1:2000 - 1:5000)
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His Tag antibody was raised against this antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His-tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His-tagged proteins tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 1% BSA, 0.01% Gentamicin sulfate
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.