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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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His Tag Antibody LS‑C123755
Antibody
His Tag antibody LS-C123755 is an unconjugated goat polyclonal antibody to His Tag. Validated for ELISA, IF, IHC and WB.
Catalog
Size
Price
LS-C123755-100
100 µg (1 mg/ml)
Unavailable

Popular His Tag Products

Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualized with FITC conjugated Mouse anti-HIS tag antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species:
Applications: IHC, Flow Cytometry
Anti-6X His Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal rabbit.
Species:
Applications: Western blot, ELISA
Antibody
Species:
Applications: Western blot, ELISA
Western blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species:
Applications: IHC, Western blot, ELISA

Product Description

His Tag antibody LS-C123755 is an unconjugated goat polyclonal antibody to His Tag. Validated for ELISA, IF, IHC and WB.
About His Tag
A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.

Specifications

Target
His Tag
Host
Goat
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1 - 2 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
His Tag antibody was raised against 6-His peptide (KLH).
Specificity
Recognizes poly-histidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a poly-histidine fusion protein expression vectors.
Usage
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA. Western Blot: 1-2 ug/ml. ELISA: 0.1-1 ug/ml. Immunohistochemistry: 1:100-1:500. Immunofluorescence: 1:100-1:500.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
100 µl Sterile ddH2O
Storage
Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/24/2018

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