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| Catalog Number | Size | Price |
|---|---|---|
| LS-C123755-100 | 100 µg | $832 |
Polyclonal Goat His Tag Antibody (IHC, IF, WB) LS‑C123755
Polyclonal Goat His Tag Antibody (IHC, IF, WB) LS‑C123755
Antibody:
His Tag Goat Polyclonal Antibody
Application:
IHC, IF, WB, ELISA
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
His Tag Goat Polyclonal Antibody
Application:
IHC, IF, WB, ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
His Tag antibody LS-C123755 is an unconjugated goat polyclonal antibody to His Tag. Validated for ELISA, IF, IHC and WB.
Host
Goat
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
6-His peptide (KLH).
Specificity
Recognizes poly-histidine fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a poly-histidine fusion protein expression vectors.
Applications
- IHC (1:100 - 1:500)
- Immunofluorescence (1:100 - 1:500)
- Western blot (1 - 2 µg/ml)
- ELISA (0.1 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA. Western Blot: 1-2 ug/ml. ELISA: 0.1-1 ug/ml. Immunohistochemistry: 1:100-1:500. Immunofluorescence: 1:100-1:500.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
sterile ddH2O.
Storage
Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Synthetic
Applications:
Immunofluorescence, Flow Cytometry
![His Tag Antibody - Anti-6X His epitope tag Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com//image2/his-tag-antibody-hrp-ls-c154274/81964_281469.jpg)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
Date Requested: 4/23/2024
