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Anti-His Tag Antibody LS-C51081

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $475
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

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Anti-His Tag Antibody (AP) LS-C67918
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Anti-His Tag Antibody (clone 33D10.D2, HRP) LS-C154015
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100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1)
Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated

Details

His Tag
Mouse
IgG1 Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry
  • ELISA

Specificity and Use

His Tag antibody was raised against synthetic peptide containing six histidine residues (6xHis) coupled to KLH.
C-terminal, N-terminal, and internal tagged fusion proteins. Antibody detects 4X, 5X and 6X tagged proteins.
Has been used in ELISA as a capture antibody.

Packaging

Lyophilized from PBS, pH 7.4, 0.02% sodium azide
100 µl deionized water
Store at -20 to -70 C. Stable for 12 months from date of receipt.
For research use only.

About His Tag

A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.

Western blot

Western blot
Comparison of THETM His Antibody, mAb, Mouse (A: LS-C51081, 1 ug/ml) with Mouse Anti-His mAb (B: Competitor B, 1 ug/ml) by Western blot. The assay was performed with cell lysates containing His-tagged fusion protein. The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody and LumiSensor HRP Substrate Kit.

Western blot

Western blot
Consistency analysis of Batch 1#, 2#, 3# and 4# of THETM His Antibody, mAb, Mouse (LS-C51081, 1 ug/ml) by Western blot, showing that signal remains consistent from Lot to Lot. The assay was performed with His-tagged fusion protein. The signal was developed with IRDye 800 Conjugated Goat Anti-Mouse IgG.

Western blot

Western blot
Consistency analysis of Batch 1#, 2#, 3# and 4# of THETM His Antibody, mAb, Mouse (LS-C51081, 1 ug/ml) by Western blot, showing that signal remains consistent from Lot to Lot. The assay was performed with His-tagged fusion protein. The signal was developed with IRDye 800 Conjugated Goat Anti-Mouse IgG.

Western blot

Western blot
Comparison of THETM His Antibody, mAb, Mouse (A: LS-C51081, 0.1 ug/ml) with Mouse Anti-His mAb (B: Competitor A, 0.1 ug/ml) by Western blot. The assay was performed with His-tagged fusion protein. The signal was developed with One-Step Western Basic Kit.

Western blot

Western blot
Western blot of His-tagged fusion proteins using THETM His Antibody, mAb, Mouse (LS-C51081, 1 ug/ml) Lane 1: N-terminal His-tagged fusion protein Lane 2: C-terminal His-tagged fusion protein The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody and LumiSensor HRP Substrate Kit.

Western blot

Western blot
Western blot of Multiple Tag Cell Lysate using THETM His Antibody, mAb, Mouse (LS-C51081, 1 ug/ml) The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody and LumiSensor HRP Substrate Kit. Predicted Size: 52 kD Observed Size: 52 kD

Western blot

Western blot
Comparison of THETM His Antibody, mAb, Mouse (A: LS-C51081, 1 ug/ml) with Mouse Anti-His mAbs (B: Competitor Q#1, 1 ug/ml; C: Competitor Q#2, 1 ug/ml) by Dot blot. The assay was performed with His-tagged fusion protein. The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody and LumiSensor HRP Substrate Kit.
 

Requested From: United States
Date Requested: 12/4/2016

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