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Anti-His Tag Antibody (clone AD1.1.10) LS-C188122


Wt. Vol. Conc. Price
2000 µg - 1 mg/ml $1,545
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

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Mouse Monoclonal [clone AD1.1.10] (IgG1)
IHC - Frozen, Western blot, Immunoprecipitation, ELISA


His Tag
IgG1 Monoclonal [AD1.1.10]
Protein G purified


  • IHC - Frozen
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation

Specificity and Use

His Tag antibody was raised against pAX6 transcription factor linked to histidine tag.
Recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian ; ; and cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. Is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.


PBS, 0.09% sodium azide
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About His Tag

A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.

Western blot

Western blot
Western blot of HIS-tagged protein probed with Mouse anti-Histidine tag visualised with Rabbit anti-Mouse IgG : HRP

Requested From: United States
Date Requested: 10/22/2016

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