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Anti-His Tag Antibody (clone 33D10.D2.G8) LS-C153999

Ordering

Wt. Vol. Conc. Price
100 µg - - $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

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Mouse Monoclonal [clone 33D10.D2.G8] (IgG1)
Western blot, ELISA
Unconjugated

Details

His Tag
Mouse
IgG1 Monoclonal [33D10.D2.G8]
Unconjugated
Purified
Unmodified

Applications

  • Western blot (1:1000)
  • ELISA

Specificity and Use

His Tag antibody was raised against hIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
6X HIS Epitope Tag antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About His Tag

A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.

Western blot

Western blot
Anti-6x-His Epitope Tag Monoclonal Antibody - Western Blot. Western blot of Immunochemicals Mouse Anti-6x-His Epitope Tag Monoclonal Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3). In lane 1 are molecular weight markers.
 

Requested From: United States
Date Requested: 12/3/2016

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