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| Catalog Number | Size | Price |
|---|---|---|
| LS-C742118-40 | 40 µg | $385 |
![His Tag Antibody - Sensitivity and specificity of MonoRab TM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit to 6xHis-tag Fusion Protein by Western blot. Loading: His tagged fusion protein Primary Antibody: MonoRabTM Anti-His Tag Antibody 0.2 µg/ml Secondary Antibody: Goat anti-rabbit IgG (H&L) [IRDye 800 ] (Licor,926-32211) 0.125 µg/ml](https://lsbio-7d62.kxcdn.com/image2/his-tag-antibody-clone-25b6e11-ls-c742118/420832_3327565.jpg)
![His Tag Antibody - Western blot analysis of cross-reactivity of MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit Primary Antibody: A: MonoRab TM Anti-His Tag Antibody 1µg/ml B: Rabbit IgG (Negative control) 1µg/ml Secondary Antibody: Goat anti-Rabbit IgG (H&L) [IRDye 800 ] (Licor,926-32211)00.125µg/ml](https://lsbio-7d62.kxcdn.com/image2/his-tag-antibody-clone-25b6e11-ls-c742118/420831_3322300.jpg)
![His Tag Antibody - Western blot analysis of immunoprecipitation from cell lysates containing His fusion protein by using MonoRabTM Anti-His Tag (C-term) Antibody (25B6E11), mAb, Rabbit Loading: Lane 1: MonoRab TM Anti-His antibody (4 µg) + His tagged fusion protein Lane 2: Rabbit IgG (4 µg) + His tagged fusion protein Lane 3: His tagged fusion protein (Input) Primary Antibody: THETM His tag antibody, mAb, Mouse 0.2 µg/ml Secondary Antibody: Goat anti-rabbit IgG [IRDye 800 ] (Licor,926-32211) 0.125 µg/ml](https://lsbio-7d62.kxcdn.com/image2/his-tag-antibody-clone-25b6e11-ls-c742118/420830_5162577.jpg)
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Monoclonal Rabbit His Tag Antibody (clone 25B6E11, IF, WB) LS‑C742118
Monoclonal Rabbit His Tag Antibody (clone 25B6E11, IF, WB) LS‑C742118
Antibody:
His Tag Rabbit Monoclonal (25B6E11) Antibody
Application:
IF, WB, IP, Flo, ELISA
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
His Tag Rabbit Monoclonal (25B6E11) Antibody
Application:
IF, WB, IP, Flo, ELISA
Format:
Unconjugated, Unmodified
Specifications
Description
His Tag antibody LS-C742118 is an unconjugated rabbit monoclonal antibody to His Tag. Validated for ELISA, Flow, IF, IP and WB.
Host
Rabbit
Clonality
IgG
Monoclonal
Clone
25B6E11
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Applications
- Immunofluorescence
- Western blot
- Immunoprecipitation
- Flow Cytometry
- ELISA
Usage
Recognizes His-tags at the C-terminus and internal regions of recombinant His-tagged proteins. Not recommended for detection of N-terminal His-tags.
Storage
Long term: store at -20°C for up to 2 years. Reconstituted: store at 2°C to 8°C for up to 3 weeks or at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Synthetic
Applications:
Immunofluorescence, Flow Cytometry
![His Tag Antibody - Anti-6X His epitope tag Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com//image2/his-tag-antibody-hrp-ls-c154274/81964_281469.jpg)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
