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Anti-His Tag Antibody (Biotin) LS-C203640

Catalog Size Price
LS-C203640-40 40 µg (0.5 mg/ml) Unavailable
LS-C203640-100 100 µg Unavailable

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Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualized with FITC conjugated Mouse anti-HIS tag antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Western blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Rabbit Polyclonal (IgG)
Western blot
Biotin Conjugated

Details

His Tag
Rabbit
IgG Polyclonal
Biotin
Immunoaffinity purified
Unmodified

Applications

Western blot (0.2 - 1 µg/ml)

Specificity and Use

His Tag antibody was raised against his epitope tag peptide HHHHHH conjugated to KLH.
Specifically reacts with fusion proteins containing His tagged fusion proteins.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Packaging

Lyophilized from PBS, pH 7.4, 1% BSA, 0.02% sodium azide.
80 µl Distilled water
Store lyophilized at -20°C. The reconstituted product can be stored for short term at 4°C or long term at -20°C or below. Avoid freeze/thaw cycles.
For research use only.

About His Tag

A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.

Western blot

Western blot of His tagged fusion protein using His-tag antibody [Biotin], pAb, Rabbit (1 ug/ml). The signal was developed with Streptavidin-HRP. Predicted Size: 27 kD. Observed Size: 27 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of His tagged fusion protein using His-tag antibody [Biotin], pAb, Rabbit (1 ug/ml). The signal was developed with Streptavidin-HRP. Predicted Size: 27 kD. Observed Size: 27 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 5/23/2018

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