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| Catalog Number | Size | Price |
|---|---|---|
| LS-C67922-100 | 100 µg | $774 |
Monoclonal Mouse His Tag Antibody (Biotin, WB) LS‑C67922
Monoclonal Mouse His Tag Antibody (Biotin, WB) LS‑C67922
Antibody:
His Tag Mouse Monoclonal (Biotin) Antibody
Application:
WB, ELISA
Format:
Biotin, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
His Tag Mouse Monoclonal (Biotin) Antibody
Application:
WB, ELISA
Format:
Biotin, Unmodified
Specifications
Description
His Tag antibody LS-C67922 is a biotin-conjugated mouse monoclonal antibody to His Tag. Validated for ELISA and WB.
Host
Mouse
Clonality
IgG1
Monoclonal
Conjugations
Biotin
Purification
Protein G purified
Modifications
Unmodified
Immunogen
PAX6 transcription factor linked to histidine tag.
Specificity
Recognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. The antibody may be used to track the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins so far tested. In Western Blot of bacterial extracts, the antibody has been shown not to cross-react with any endogenous products. Some cross-reactivity may be seen with extracts of insect or mammalian cells.
Applications
- Western blot
- ELISA (1:500)
Usage
Suitable for use in ELISA and Western Blot. Some cross-reactivity may be seen with extracts of insect or mammalian cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4, 0.09% sodium azide, 40% glycerol.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Synthetic
Applications:
Immunofluorescence, Flow Cytometry
![His Tag Antibody - Anti-6X His epitope tag Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com//image2/his-tag-antibody-hrp-ls-c154274/81964_281469.jpg)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
Date Requested: 4/24/2024
