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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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His Tag Antibody (Biotin) LS‑C187773
Antibody
His Tag antibody LS-C187773 is a biotin-conjugated goat polyclonal antibody to His Tag. Validated for ELISA and WB.
Catalog
Size
Price
LS-C187773-100
100 µg (1 mg/ml)
Unavailable

Popular His Tag Products

Human anti-human CD5 (HuCAL dHLX antibody with HIS tag) staining human peripheral blood lymphocytes, visualized with FITC conjugated Mouse anti-HIS tag antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species:
Applications: IHC, Flow Cytometry
Anti-6X His Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal rabbit.
Species:
Applications: Western blot, ELISA
Antibody
Species:
Applications: Western blot, ELISA
Western blot of Mouse anti-6xHIS Tag Antibody. Lane 1: 100ng Purified histidine-tagged recombinant protein. Lane 2: 200ng E. coli cell lysate containing histidine-tagged expression construct. Lane 3: 100ng Purified GST-tagged recombinant protein. Lane 4: 100ng Purified FLAG-tagged recombinant protein. Primary antibody: Mouse anti-6xHIS Tag antibody at 1:5000 overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20000 for 30 min at RT. Block: 5% BLOTTO for 1 hr at RT. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species:
Applications: IHC, Western blot, ELISA

Product Description

His Tag antibody LS-C187773 is a biotin-conjugated goat polyclonal antibody to His Tag. Validated for ELISA and WB.
About His Tag
A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.

Specifications

Target
His Tag
Host
Goat
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:10000 - 1:100000)
  • ELISA (1:10000 - 1:100000)
Immunogen
His Tag antibody was raised against keyhole limpet hemocyanin (KLH) conjugated H-H-H-H-H-H.
Specificity
Recognizes the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. May not detect all N-Terminal (NT) histidine tagged proteins. Has been shown to be suitable for use on cytospins.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.09% sodium azide, 0.2% BSA
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/23/2018

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