Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Hypoxia Signaling Cascade for Erythropoietin Production in Hepatocytes. Tojo Y, Sekine H, Hirano I, Pan X, Souma T, Tsujita T, Kawaguchi S, Takeda N, Takeda K, Fong GH, Dan T, Ichinose M, Miyata T, Yamamoto M, Suzuki N. Molecular and cellular biology. 2015 35:2658-72. (WB; Human)[Full Text Article]
EPAS1 Antibody, BHLHe73 Antibody, ECYT4 Antibody, HIF-1-alpha-like factor Antibody, HIF2-alpha Antibody, HIF2A Antibody, EPAS-1 Antibody, HIF-2-alpha Antibody, HIF-1alpha-like factor Antibody, MOP2 Antibody, Member of PAS protein 2 Antibody, PASD2 Antibody
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier.
Detection of Human, Mouse, and Rat HIF-2 alpha/EPAS1 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, bEnd.3 mouse endothelioma cell line, and PC-12 rat adrenal pheochromocytoma cell line untreated(-) or treated (+) with 150 uM CoCl2 for 16 hours. PVDF Membrane was probed with 1 ug/ml of Mouse HIF-2 alpha/EPAS1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for HIF-2 alpha/EPAS1 at approximately 120 kD (as indicated). This experiment was conducted under reducing conditions.