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Anti-HIF2A / EPAS1 Antibody (aa535-631, clone ep190b) IHC-plus™ LS-B501

Note: This antibody replaces LS-C2564, LS-C7475
Catalog Size Price
LS-B501-100 100 µl (1 mg/ml) Unavailable

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Anti-HIF2A / EPAS1 Antibody (aa535-631, clone ep190b) IHC-plus™ LS-B501
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Immunohistochemistry Image
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Anti-HIF2A / EPAS1 Antibody (C-Terminus) LS-C123798
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Western blot Image

100% Guaranteed Publications (2)
Mouse Monoclonal [clone ep190b] (IgG1) to Human HIF2A / EPAS1
Human, Mouse, Rat
IHC - Paraffin, Western blot (applications tested for the base form of this product only)


Human HIF2A / EPAS1
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [ep190b]
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Protein G purified


  • IHC - Paraffin (10 - 20 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

HIF2A / EPAS1 antibody was raised against human HIF-2 alpha amino acids 535-631
HIF-2 alpha and does not cross-react with HIF-1 alpha.
Immunohistochemistry: LS-B501 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B501 was determined to be 10-20 ug/ml.


Supplied in PBS, 0.1% sodium azide.
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
For research use only.

Publications (2)

Silencing of int6 gene restores function of the ischaemic hindlimb in a rat model of peripheral arterial disease. Okamoto N, Tanaka A, Jung K, Karasawa K, Orito K, Matsuda A, Amagai Y, Oida K, Ohmori K, Matsuda H. Cardiovascular research. 2011 Nov;92:209-17. [Full Text Article] [PubMed:21771896]
Mitochondrial respiration and redox coupling in articular chondrocytes. Lane RS, Fu Y, Matsuzaki S, Kinter M, Humphries KM, Griffin TM. Arthritis research & therapy. 2015 17:54. (WB; Human) [Full Text Article] [PubMed:25889867] [PMC:PMC4384316]

About HIF2A / EPAS1

Q99814 NM_001430 NP_001421.2

EPAS1 Antibody, BHLHe73 Antibody, ECYT4 Antibody, HIF-1-alpha-like factor Antibody, HIF2-alpha Antibody, HIF2A Antibody, EPAS-1 Antibody, HIF-2-alpha Antibody, HIF-1alpha-like factor Antibody, MOP2 Antibody, Member of PAS protein 2 Antibody, PASD2 Antibody

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier.


Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B501 concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 3/18/2018

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