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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HIF2A / EPAS1 Antibody (aa535‑631, clone Ep190b, DY488) LS‑C140827
EPAS1 antibody LS-C140827 is a DY488-conjugated mouse monoclonal antibody to EPAS1 (HIF2A) from human, mouse and rat. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C140827-100
100 µl
Unavailable

Popular HIF2A / EPAS1 Products

Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B501 concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B517 concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: IHC, IHC - Paraffin
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Species: Human, Mouse, Rat
Applications: IHC, Western blot, Immunoprecipitation
Antibody
Species: Human
Applications: IHC, IHC - Paraffin

Product Description

EPAS1 antibody LS-C140827 is a DY488-conjugated mouse monoclonal antibody to EPAS1 (HIF2A) from human, mouse and rat. Validated for Flow, IHC and WB.
About HIF2A / EPAS1
Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Q99814 NM_001430 NP_001421.2

EPAS1 Antibody, BHLHe73 Antibody, ECYT4 Antibody, HIF-1-alpha-like factor Antibody, HIF2-alpha Antibody, HIF2A Antibody, EPAS-1 Antibody, HIF-2-alpha Antibody, HIF-1alpha-like factor Antibody, MOP2 Antibody, Member of PAS protein 2 Antibody, PASD2 Antibody


Specifications

Target
Human HIF2A / EPAS1
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [Ep190b]
Conjugations
DyLight 488. Also available Unconjugated or conjugated with Biotin, DY650, DY550.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150 - 1:300)
  • Western blot (1:500)
  • Flow Cytometry (1:500)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HIF2A / EPAS1 antibody was raised against human HIF-2 alpha amino acids 535-631
Epitope
aa535-631
Specificity
HIF-2 alpha and does not cross-react with HIF-1 alpha.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM sodium borate
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B501 concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B501 concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HIF-2 alpha detected in hypoxic human lysate. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HIF-2 alpha detected in hypoxic human lysate. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B501 concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B501 concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HIF-2 alpha detected in hypoxic human lysate. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HIF-2 alpha detected in hypoxic human lysate. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B501 concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B501 concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HIF-2 alpha detected in hypoxic human lysate. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HIF-2 alpha detected in hypoxic human lysate. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B501 concentration 20 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF2A antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B501 concentration 20 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

HIF-2 alpha detected in hypoxic human lysate. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
HIF-2 alpha detected in hypoxic human lysate. Lane 1: normoxic A549 lysate control, lane 2: hypoxic A549 lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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