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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HIF1AN Antibody (N‑Terminus) LS‑C30835
HIF1AN antibody LS-C30835 is an unconjugated rabbit polyclonal antibody to HIF1AN from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C30835-100
100 µg (1 mg/ml)
Unavailable

Popular HIF1AN Products

Anti-HIF1AN antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B667 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Anti-HIF1AN antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1535 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Western analysis of fetal muscle lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications: Western blot
FIH visualized using HIF1AN antibody, tested on mouse back skin sections.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot
Immunohistochemistry of paraffin-embedded human gastric cancer tissue.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, ChIP-sequencing

Product Description

HIF1AN antibody LS-C30835 is an unconjugated rabbit polyclonal antibody to HIF1AN from human, mouse, rat and other species. Validated for WB.
About HIF1AN
Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Q9NWT6 NM_017902 NP_060372.2

HIF1AN Antibody, Factor inhibiting HIF-1 Antibody, FIH1 Antibody, Factor inhibiting HIF1 Antibody, FIH-1 Antibody


Specifications

Target
Human HIF1AN
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Bat, Horse, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1.25 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
HIF1AN antibody was raised against synthetic peptide from N-Terminus of human HIF1AN (Q9NWT6, NP_060372). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Opossum, Guinea pig (100%); Bat, Horse, Turkey, Zebra finch, Chicken (92%).
Blocking Peptide
LS-E11585 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C30835. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human HIF1AN
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
100 µl After adding water, will consist of PBS buffer with 2% sucrose
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Western blot

A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 7/17/2018

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