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HIF1AN

hypoxia inducible factor 1, alpha subunit inhibitor

Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.

Gene Name: hypoxia inducible factor 1, alpha subunit inhibitor
Synonyms: HIF1AN, Factor inhibiting HIF-1, FIH1, Factor inhibiting HIF1, FIH-1
Target Sequences: NM_017902 NP_060372.2 Q9NWT6

Publications (15)

1
Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (FIH) in regulating hypoxia-inducible factor (HIF) transcriptional target genes [corrected]. Stolze IP, Tian YM, Appelhoff RJ, Turley H, Wykoff CC, Gleadle JM, Ratcliffe PJ. The Journal of biological chemistry. 2004 279:42719-25. [PubMed:15302861]
2
Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues. Soilleux EJ, Turley H, Tian YM, Pugh CW, Gatter KC, Harris AL. Histopathology. 2005 47:602-10. [PubMed:16324198]
3
Posttranslational hydroxylation of ankyrin repeats in IkappaB proteins by the hypoxia-inducible factor (HIF) asparaginyl hydroxylase, factor inhibiting HIF (FIH). Cockman ME, Lancaster DE, Stolze IP, Hewitson KS, McDonough MA, Coleman ML, Coles CH, Yu X, Hay RT, Ley SC, Pugh CW, Oldham NJ, Masson N, Schofield CJ, Ratcliffe PJ. Proceedings of the National Academy of Sciences of the United States of America. 2006 103:14767-72. [PubMed:17003112] [PMC:PMC1578504]
4
ASB4 is a hydroxylation substrate of FIH and promotes vascular differentiation via an oxygen-dependent mechanism. Ferguson JE, Wu Y, Smith K, Charles P, Powers K, Wang H, Patterson C. Molecular and cellular biology. 2007 27:6407-19. [PubMed:17636018] [PMC:PMC2099627]
5
Protein kinase C-mediated modulation of FIH-1 expression by the homeodomain protein CDP/Cut/Cux. Li J, Wang E, Dutta S, Lau JS, Jiang SW, Datta K, Mukhopadhyay D. Molecular and cellular biology. 2007 27:7345-53. [PubMed:17682059] [PMC:PMC2168911]
6
Interaction with factor inhibiting HIF-1 defines an additional mode of cross-coupling between the Notch and hypoxia signaling pathways. Zheng X, Linke S, Dias JM, Zheng X, Gradin K, Wallis TP, Hamilton BR, Gustafsson M, Ruas JL, Wilkins S, Bilton RL, Brismar K, Whitelaw ML, Pereira T, Gorman JJ, Ericson J, Peet DJ, Lendahl U, Poellinger L. Proceedings of the National Academy of Sciences of the United States of America. 2008 105:3368-73. (WB) [PubMed:18299578] [PMC:PMC2265116]
7
The basic cleft of RPA70N binds multiple checkpoint proteins, including RAD9, to regulate ATR signaling. Xu X, Vaithiyalingam S, Glick GG, Mordes DA, Chazin WJ, Cortez D. Molecular and cellular biology. 2008 28:7345-53. [PubMed:18936170] [PMC:PMC2593429]
8
A feedback loop involving the Phd3 prolyl hydroxylase tunes the mammalian hypoxic response in vivo. Minamishima YA, Moslehi J, Padera RF, Bronson RT, Liao R, Kaelin WG. Molecular and cellular biology. 2009 29:5729-41. (WB; Mouse) [PubMed:19720742] [PMC:PMC2772748]
9
BRCA1 tumours correlate with a HIF-1alpha phenotype and have a poor prognosis through modulation of hydroxylase enzyme profile expression. Yan M, Rayoo M, Takano EA, Thorne H, Fox SB. British journal of cancer. 2009 101:1168-74. [PubMed:19724277] [PMC:PMC2768103]
10
Factor inhibiting HIF (FIH-1) promotes renal cancer cell survival by protecting cells from HIF-1-mediated apoptosis. Khan MN, Bhattacharyya T, Andrikopoulos P, Esteban MA, Barod R, Connor T, Ashcroft M, Maxwell PH, Kiriakidis S. British journal of cancer. 2011 104:1151-9. (WB; Human) [PubMed:21386837] [PMC:PMC3068507]
11
Quantitative mass spectrometry reveals dynamics of factor-inhibiting hypoxia-inducible factor-catalyzed hydroxylation. Singleton RS, Trudgian DC, Fischer R, Kessler BM, Ratcliffe PJ, Cockman ME. The Journal of biological chemistry. 2011 286:33784-94. [PubMed:21808058] [PMC:PMC3190818]
12
Synthetic transactivation screening reveals ETV4 as broad coactivator of hypoxia-inducible factor signaling. Wollenick K, Hu J, Kristiansen G, Schraml P, Rehrauer H, Berchner-Pfannschmidt U, Fandrey J, Wenger RH, Stiehl DP. Nucleic acids research. 2012 40:1928-43. [PubMed:22075993] [PMC:PMC3300025]
13
Factor inhibiting HIF (FIH) recognizes distinct molecular features within hypoxia-inducible factor- (HIF-) versus ankyrin repeat substrates. Wilkins SE, Karttunen S, Hampton-Smith RJ, Murchland I, Chapman-Smith A, Peet DJ. The Journal of biological chemistry. 2012 287:8769-81. [PubMed:22270367] [PMC:PMC3308777]
14
Increased activation of the hypoxia-inducible factor pathway in varicose veins. Lim CS, Kiriakidis S, Paleolog EM, Davies AH. Journal of vascular surgery. 2012 55:1427-39. (WB; Human) [PubMed:22277691]
15
MT1-MMP plays a critical role in hematopoiesis by regulating HIF-mediated chemokine/cytokine gene transcription within niche cells. Nishida C, Kusubata K, Tashiro Y, Gritli I, Sato A, Ohki-Koizumi M, Morita Y, Nagano M, Sakamoto T, Koshikawa N, Kuchimaru T, Kizaka-Kondoh S, Seiki M, Nakauchi H, Heissig B, Hattori K. Blood. 2012 119:5405-16. [PubMed:22544701]
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HIF1AN Antibody - Anti-HIF1AN antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHC-plus™ HIF1AN Antibody (C-Terminus) LS-B667
(4.0) Rating
HIF1AN Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Mouse, Rat, Human
IHC, IHC-P, IP, WB
Unconjugated
0.1 ml/$460
HIF1AN Antibody - Anti-HIF1AN antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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HIF1AN Antibody (C-Terminus, Biotin) LS-C141144
HIF1AN Rabbit anti-Human Polyclonal (C-Terminus) (Biotin) Antibody
Mouse, Rat, Human
IHC, IHC-P, IP, WB
Biotin Conjugated
0.1 ml/$416
HIF1AN Antibody - Anti-HIF1AN antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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HIF1AN Antibody (C-Terminus, DY650) LS-C141145
HIF1AN Rabbit anti-Human Polyclonal (C-Terminus) (DY650) Antibody
Mouse, Rat, Human
IHC, IHC-P, IP, WB
DY650 Conjugated
0.1 ml/$416
HIF1AN Antibody - Anti-HIF1AN antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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HIF1AN Antibody (C-Terminus, DY488) LS-C141146
HIF1AN Rabbit anti-Human Polyclonal (C-Terminus) (DY488) Antibody
Mouse, Rat, Human
IHC, IHC-P, IP, WB
DY488 Conjugated
0.1 ml/$416
HIF1AN Antibody - Anti-HIF1AN antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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HIF1AN Antibody (C-Terminus, DY550) LS-C141148
HIF1AN Rabbit anti-Human Polyclonal (C-Terminus) (DY550) Antibody
Mouse, Rat, Human
IHC, IHC-P, IP, WB
DY550 Conjugated
0.1 ml/$416
HIF1AN Antibody - Anti-HIF1AN antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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HIF1AN Antibody (N-Terminus) LS-B1535
HIF1AN Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
HIF1AN Antibody - Immunohistochemistry of paraffin-embedded rat brain tissue.
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HIF1AN Antibody LS-C334077
HIF1AN Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ChIP-Seq, IF, IHC, IP, WB
Unconjugated
20 µl/$287; 50 µl/$341; 100 µl/$431; 200 µl/$539
HIF1AN Antibody - Immunohistochemical analysis of FIH-1 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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HIF1AN Antibody LS-C482449
HIF1AN Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ChrIP, ICC, IF, IHC, IHC-P, IP, WB
Unconjugated
50 µl/$286; 100 µl/$321; 200 µl/$386
HIF1AN Antibody - Western analysis of fetal muscle lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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HIF1AN Antibody (C-Terminus) LS-C101749
HIF1AN Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Mouse, Human
WB
Unconjugated
100 µl/$424
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HIF1AN Antibody LS-C129762
HIF1AN Rabbit anti-Human Monoclonal Antibody
Mouse, Rat, Human, Monkey
WB
Unconjugated
100 µl/$688
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HIF1AN Antibody LS-C755054
HIF1AN Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IP, WB
Unconjugated
100 µg/$331
HIF1AN Antibody - Factor Inhibiting HIF-1 Antibody (162c) - IHC of FIH in human renal cancer using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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HIF1AN Antibody (clone 162c) LS-C143194
HIF1AN Mouse anti-Human Monoclonal (162c) Antibody
Human
ICC, IHC, IHC-P, WB
Unconjugated
0.1 ml/$386
HIF1AN Antibody - Factor Inhibiting HIF-1 Antibody (162c) - IHC of FIH in human renal cancer using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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HIF1AN Antibody (clone 162c, Biotin) LS-C143195
HIF1AN Mouse anti-Human Monoclonal (Biotin) (162c) Antibody
Human
ICC, IHC, IHC-P, WB
Biotin Conjugated
0.1 ml/$416
HIF1AN Antibody - Factor Inhibiting HIF-1 Antibody (162c) - IHC of FIH in human renal cancer using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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HIF1AN Antibody (clone 162c, DY488) LS-C143197
HIF1AN Mouse anti-Human Monoclonal (DY488) (162c) Antibody
Human
ICC, IHC, IHC-P, WB
DY488 Conjugated
0.1 ml/$416
HIF1AN Antibody - Factor Inhibiting HIF-1 Antibody (162c) - IHC of FIH in human renal cancer using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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HIF1AN Antibody (clone 162c, DY650) LS-C143196
HIF1AN Mouse anti-Human Monoclonal (DY650) (162c) Antibody
Human
ICC, IHC, IHC-P, WB
DY650 Conjugated
0.1 ml/$416
HIF1AN Antibody - Factor Inhibiting HIF-1 Antibody (162c) - IHC of FIH in human renal cancer using DAB with hematoxylin counterstain. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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HIF1AN Antibody (clone 162c, DY550) LS-C143199
HIF1AN Mouse anti-Human Monoclonal (DY550) (162c) Antibody
Human
ICC, IHC, IHC-P, WB
DY550 Conjugated
0.1 ml/$416
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HIF1AN Antibody LS-C767165
HIF1AN Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, IP, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
HIF1AN Protein - Recombinant Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor By SDS-PAGE
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Human HIF1AN Protein (Recombinant His, N-Terminal) (aa2-349) - LS-G25716
E. coli
His, N-Terminal
43.9 kDa
10 µg/$281; 50 µg/$442; 100 µg/$705; 200 µg/$882; 1 mg/$1,989; 500 µg/$1,529; 5 mg/$3,081; 2 mg/$2,176
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HIF1AN Antibody LS-C308237
HIF1AN Mouse anti-Human Monoclonal Antibody
Human
ELISA, IF, WB
Unconjugated
100 µg/$705
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HIF1AN Antibody (Biotin) LS-C522380
HIF1AN Mouse anti-Human Monoclonal (Biotin) Antibody
Human
ELISA, IF, WB
Biotin Conjugated
100 µl/$904
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HIF1AN Antibody (PE) LS-C650624
HIF1AN Mouse anti-Human Monoclonal (PE) Antibody
Human
ELISA, IF, WB
PE Conjugated
100 µl/$904
HIF1AN Antibody - Immunohistochemistry of paraffin-embedded Human esophagus cancer using HIF1AN Polyclonal Antibody at dilution of 1:40.
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HIF1AN Antibody LS-C403473
HIF1AN Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
HIF1AN Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling HIF1AN with HIF1AN Antibody (C-term) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NH174309) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
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HIF1AN Antibody (C-Terminus, clone 931CT3.1.1) LS-C757864
HIF1AN Mouse anti-Human Monoclonal (C-Terminus) (931CT3.1.1) Antibody
Human
ELISA, IF, WB
Unconjugated
200 µl/$393
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HIF1AN Antibody (Preservative Free) LS-C815335
HIF1AN Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Human
ICC, IF, IHC-P
Unconjugated, Preservative Free
100 µl/$412
HIF1AN Antibody - Immunohistochemistry of paraffin-embedded human kidney tissue using HIF1AN Antibody at dilution of 1:100
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HIF1AN Antibody LS-C379424
HIF1AN Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
50 µg/$294; 100 µg/$360
Viewing 1-25 of 84 product results



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