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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HIF1A / HIF1 Alpha Antibody (aa432‑528, clone H1alpha67, Biotin) LS‑C140776
HIF1 Alpha antibody LS-C140776 is a biotin-conjugated mouse monoclonal antibody to HIF1 Alpha (HIF1A) from human, mouse, rat and other species. Validated for ChrIP, ELISA, Flow, IF, IHC, IP and WB.
Catalog
Size
Price
LS-C140776-100
100 µl (1.2 mg/ml)
Unavailable

Popular HIF1A / HIF1 Alpha Products

Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B110 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Ferret, Pig, Rabbit, Sheep, Primate
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry, ELISA, Chromatin Immunoprecipitation
Anti-HIF1A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B495 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Rat, Goat, Hamster, Rabbit, Primate
Applications: IHC, IHC - Paraffin
Anti-HIF1A antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B674 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-HIF1A antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1503 concentration 10 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig
Applications: IHC, IHC - Paraffin, Western blot
Anti-HIF1A antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2823 dilution 1:100.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Dog, Guinea pig, Xenopus, Zebrafish, Primate
Applications: IHC, IHC - Paraffin

Product Description

HIF1 Alpha antibody LS-C140776 is a biotin-conjugated mouse monoclonal antibody to HIF1 Alpha (HIF1A) from human, mouse, rat and other species. Validated for ChrIP, ELISA, Flow, IF, IHC, IP and WB.
About HIF1A / HIF1 Alpha
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Q16665 NM_001530 NP_001521.1

HIF1A Antibody, ARNT-interacting protein Antibody, ARNT interacting protein Antibody, BHLHe78 Antibody, HIF1 Alpha Antibody, Hypoxia-inducible factor1alpha Antibody, HIF-1A Antibody, HIF1 Antibody, MOP1 Antibody, Member of PAS superfamily 1 Antibody, PASD8 Antibody, HIF-1-alpha Antibody, HIF-1alpha Antibody, HIF1-ALPHA Antibody, Member of PAS protein 1 Antibody


Specifications

Target
Human HIF1A / HIF1 Alpha
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Ferret, Pig, Rabbit, Sheep, Xenopus, Yeast, Primate (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [H1alpha67]
Conjugations
Biotin. Also available Unconjugated or conjugated with DY650, DY488, DY550.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Immunofluorescence
  • Western blot (1:500)
  • Immunoprecipitation
  • Flow Cytometry
  • ELISA
  • Chromatin Immunoprecipitation
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Fusion protein containing amino acids 432-528 of human HIF-1alpha
Epitope
aa432-528
Specificity
HIF-1 alpha.
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.05% sodium azide
Storage
Store at 4°C. Do not freeze. Protect from light.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

HIF-1 alpha Antibody (H1alpha67) - IHC of human glioblastoma multiforme. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HIF-1 alpha Antibody (H1alpha67) - IHC of human glioblastoma multiforme. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B110 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B110 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemical staining of human glioblastoma multiforme.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemical staining of human glioblastoma multiforme. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of 50ug cobalt chloride induced COS-7 nuclear extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of 50ug cobalt chloride induced COS-7 nuclear extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

HIF-1 alpha Antibody (H1alpha67) - IHC of human glioblastoma multiforme. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HIF-1 alpha Antibody (H1alpha67) - IHC of human glioblastoma multiforme. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B110 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B110 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemical staining of human glioblastoma multiforme.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemical staining of human glioblastoma multiforme. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of 50ug cobalt chloride induced COS-7 nuclear extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of 50ug cobalt chloride induced COS-7 nuclear extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

HIF-1 alpha Antibody (H1alpha67) - IHC of human glioblastoma multiforme. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HIF-1 alpha Antibody (H1alpha67) - IHC of human glioblastoma multiforme. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B110 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B110 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemical staining of human glioblastoma multiforme.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemical staining of human glioblastoma multiforme. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of 50ug cobalt chloride induced COS-7 nuclear extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of 50ug cobalt chloride induced COS-7 nuclear extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

HIF-1 alpha Antibody (H1alpha67) - IHC of human glioblastoma multiforme. This image was taken for the unconjugated form of this product. Other forms have not been tested.
HIF-1 alpha Antibody (H1alpha67) - IHC of human glioblastoma multiforme. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B110 concentration 10 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HIF1A antibody IHC of human kidney cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B110 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemical staining of human glioblastoma multiforme.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Immunohistochemical staining of human glioblastoma multiforme. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western analysis of 50ug cobalt chloride induced COS-7 nuclear extracts.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western analysis of 50ug cobalt chloride induced COS-7 nuclear extracts. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/17/2018

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