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Catalog Number	Size	Price
LS-C186512-100	100 µg (0.5 mg/ml)	$454

HIC1 Antibody - HIC1 antibody (1 ug/ml) staining of A549 (A) and Jurkat (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HIC1 Antibody - HIC1 antibody HEK293 overexpressing Human HIC1 and probed with (0.5 ug/ml) with the mock transfection in first lane. Data obtained from Dr. D. Leprince, CNRS UMR 8161, Institut de Biologie de LILLE, France

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Polyclonal Goat anti‑Human HIC1 Antibody (aa396‑410, WB) LS‑C186512

Name: Anti-HIC1 Antibody | Goat anti-Human Polyclonal Peptide-ELISA,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C186512-100
Price: 454.0000 USD
Availability: InStock

Submit Review LSBio Guarantee

Antibody:

HIC1 Goat anti-Human Polyclonal (aa396-410) Antibody

Application:

WB, Peptide-ELISA

Reactivity:

Human, Bovine, Dog, Hamster, Pig

Format:

Unconjugated, Unmodified

$454

LS-C186512-100

Toll Free North America

206-374-1102

For Research Use Only

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Overview

Antibody:

HIC1 Goat anti-Human Polyclonal (aa396-410) Antibody

Application:

WB, Peptide-ELISA

Reactivity:

Human, Bovine, Dog, Hamster, Pig

Format:

Unconjugated, Unmodified

Specifications

Description

HIC1 antibody LS-C186512 is an unconjugated goat polyclonal antibody to HIC1 (aa396-410) from human. It is reactive with human, bovine, dog and other species. Validated for Peptide-ELISA and WB.

Target

Human HIC1

Synonyms

HIC1 | Hic-1 | ZNF901 | Hypermethylated in cancer 1 | ZBTB29

See All HIC1 Antibodies

Host

Goat

Reactivity

Human, Bovine, Dog, Hamster, Pig (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence HLEGYPCPHLAYGEP, from the internal region of the protein sequence according to NP_006488.2NP_001091672.1.

Epitope

aa396-410

Specificity

Human HIC1. This antibody is expected to recognize both reported isoforms (NP_006488.2; NP_001091672.1).

Applications

Western blot (0.5 - 1 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:128000)

Usage

Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kD is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kD according to NP_006488.2. Recommended concentration, 0.5-1 ug/ml.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About HIC1

Uniprot: Q14526 NCBI: NM_006497 NP_006488.2