Target
Human HIC1
Synonyms
HIC1 | Hic-1 | ZNF901 | Hypermethylated in cancer 1 | ZBTB29
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence NTHCAELYASGP, from the internal region of the protein sequence according to NP_006488.2NP_001091672.1.
Specificity
Human HIC1. This antibody is expected to recognize both reported isoforms (NP_006488.2; NP_001091672.1).
Applications
- Western blot (0.5 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Human HIC1 a band of approx. 100kD is observed. This band is not observed in the mock-transfected HEK293. The same band is observed endogenously in the cell lines A549 and Jurkat. The calculated molecular size is 74.5kD according to NP_006488.2. Recommended concentration, 0.5-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee