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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HFE2 / Hemojuvelin Antibody (clone 1C12) IHC‑plus™ LS‑B7222 Best Seller
Note: This antibody replaces LS-C134124
Hemojuvelin antibody LS-B7222 is an unconjugated mouse monoclonal antibody to human Hemojuvelin (HFE2). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B7222-50
50 µg (0.5 mg/ml)
Unavailable

Popular HFE2 / Hemojuvelin Products

Western blot of HFE2 Antibody in HepG2 cell line lysates (35 ug/lane). HFE2 (arrow) was detected using the purified antibody.
Species: Human
Applications: Western blot, Flow Cytometry
Antibody
Species: Human
Applications: Western blot, Flow Cytometry, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Western blot of recombinant HFE2 / Hemojuvelin.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot

Product Description

Hemojuvelin antibody LS-B7222 is an unconjugated mouse monoclonal antibody to human Hemojuvelin (HFE2). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About HFE2 / Hemojuvelin
HFE2 / Hemojuvelin is involved in iron metabolism. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. It could also represent the cellular receptor for hepcidin. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Defects in this gene are the cause of hemochromatosis type 2A, also called juvenile hemochromatosis (JH). Q6ZVN8 NP_973733.1

HFE2 Antibody, Haemojuvelin Antibody, HFE2A Antibody, HJV Antibody, Hemochromatosis type 2 protein Antibody, Repulsive guidance molecule c Antibody, RGM domain family member C Antibody, Hemojuvelin Antibody, JH Antibody, RGMC Antibody


Specifications

Target
Human HFE2 / Hemojuvelin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [1C12]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HFE2 (NP_973733, 93 a.a. ~ 174 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human HFE2
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
Presentation
PBS, pH 7.4. Sourced in Ascites.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-HFE2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7222 concentration 5 ug/ml.
Anti-HFE2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7222 concentration 5 ug/ml.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HFE2 is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HFE2 is 3 ng/ml as a capture antibody.

Immunohistochemistry

Anti-HFE2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7222 concentration 5 ug/ml.
Anti-HFE2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7222 concentration 5 ug/ml.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HFE2 is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HFE2 is 3 ng/ml as a capture antibody.

Immunohistochemistry

Anti-HFE2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7222 concentration 5 ug/ml.
Anti-HFE2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7222 concentration 5 ug/ml.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HFE2 is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HFE2 is 3 ng/ml as a capture antibody.

Immunohistochemistry

Anti-HFE2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7222 concentration 5 ug/ml.
Anti-HFE2 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7222 concentration 5 ug/ml.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HFE2 is 3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HFE2 is 3 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/23/2018

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