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Anti-HES4 Antibody (aa94-221) LS-C36653

Ordering

Wt. Vol. Conc. Price
100 µg 100 µl - $650
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular HES4 Antibodies

Anti-HES4 Antibody (aa94-221) LS-C36653
Sheep Polyclonal (IgG) to Human HES4
Human
ICC, Western blot, ELISA
Unconjugated
Anti-HES4 Antibody (aa94-221, Biotin) LS-C36654
Sheep Polyclonal (IgG) to Human HES4
Human
Western blot
Biotin Conjugated
Anti-HES4 Antibody IHC-plus™ LS-B3076
Rabbit Polyclonal (IgG) to Human HES4
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human HES4
Human
ICC, Western blot, ELISA
Unconjugated

Details

Human HES4
Sheep
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Affinity purified
Unmodified

Applications

  • ICC
  • Western blot
  • ELISA

Specificity and Use

HES4 antibody was raised against e. coli-derived rhHES-4 (aa94-221).
aa94-221
Selected for its ability to recognize human HES-4 in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 1% cross-reactivity with rhHES-1, rhHES-2, rhHES-5, and rhHES-7.
Western Blot: This antibody can be used at 0.1-0.2 ug/ml with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 ug/ml to detect HES-4 in HEK293 cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA. Direct ELISA: This antibody can be used at 0.5-1.0 ug/ml with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 0.2 ng/well.

Packaging

Lyophilized.
100 µl deionized water
For research use only.

About HES4

Q9HCC6 NM_021170 NP_066993.1

HES4 Antibody, BHLHb42 Antibody, Transcription factor HES-4 Antibody, BHLH factor Hes4 Antibody, Hairy and enhancer of split 4 Antibody, HHES4 Antibody

Transcriptional repressor. Binds DNA on N-box motifs: 5'-CACNAG-3'.

Requested From: United States
Date Requested: 12/3/2016

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