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Anti-HEPH / Hephaestin Antibody (C-Terminus) LS-C24201

Catalog Size Price
LS-C24201-50 50 µg (1 mg/ml) Unavailable

Most Popular HEPH / Hephaestin Antibodies

Anti-HEPH / Hephaestin Antibody (C-Terminus) LS-C24201
Rabbit Polyclonal (IgG) to Mouse HEPH / Hephaestin
Mouse, Rat
Western blot, ELISA
Unconjugated
Anti-HEPH / Hephaestin Antibody (C-Terminus) LS-C24202
Rabbit Polyclonal (IgG) to Mouse HEPH / Hephaestin
Mouse
Western blot, ELISA
Unconjugated
Anti-HEPH / Hephaestin Antibody (aa315-425, clone 10D112, Azide-free) LS-C183684
Mouse Monoclonal [clone 10D112] (IgG2a) to Human HEPH / Hephaestin
Human
Western blot, ELISA
Unconjugated, Azide-free
Anti-HEPH / Hephaestin Antibody (aa24-366, FITC) LS-C424620
Rabbit Polyclonal (IgG) to Human HEPH / Hephaestin
Human
ICC, Western blot, ELISA
FITC Conjugated
Anti-HEPH / Hephaestin Antibody (aa24-366, Biotin) LS-C424621
Rabbit Polyclonal (IgG) to Human HEPH / Hephaestin
Human
ICC, Western blot, ELISA
Biotin Conjugated

100% Guaranteed
Rabbit Polyclonal (IgG) to Mouse HEPH / Hephaestin
Mouse, Rat
Western blot, ELISA
Unconjugated

Details

Mouse HEPH / Hephaestin
Rabbit
Mouse, Rat (tested or 100% immunogen sequence identity)
Human (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:1000 - 1:5000)
  • ELISA

Specificity and Use

HEPH / Hephaestin antibody was raised against an 18-aa peptide sequence within the C-terminus of mouse/rat Hp (1) was synthesized, coupled to KLH.
C-Terminus
The mouse sequence is 100% conserved in rat and 94% in human Hp. Control peptide, because of the small size (2-3kD), is not recommended for Western. It should be used in ELISA or antibody blocking (use 5-10 ug control peptide per 1 ug of IgG or 1 ul of antiserum) experiments to demonstrate antibody specificity.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for antiserum and 1-10 ug/ml for aff. pure IgG using ECL. ELISA: ~1:100000 using 50-100 ng control peptide/well.

Packaging

PBS, pH 7.4, 0.1% BSA.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About HEPH / Hephaestin

Q9BQS7 NM_014799 NP_055614.1

HEPH Antibody, Hephaestin Antibody, CPL Antibody, KIAA0698 Antibody

May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1.

Requested From: 
Date Requested: 6/27/2017

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