Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 3F6-G10] (IgG2a) to Hepatitis B Virus Hepatitis B x Antigen (HBxAg)
Hepatitis B Virus
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Hepatitis B Virus Hepatitis B x Antigen (HBxAg)
Hepatitis B Virus (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [3F6-G10]
IHC - Paraffin (1:100)
IHC - Frozen (1:100)
Western blot (1:100 - 1:500)
Specificity and Use
HB-Xag-Protein A Fusion protein.
recognizes the HB-X antigen of hepatitis virus. The Hepatitis B X antigen is a 154 amino acid ~17 kD multifunctional protein involved in the development of liver cirrhosis and hepatocellular carcinoma. Clone 3F6-G10 was produced by immunization of mice with a "HB-X- Protein A" fusion construct and subsequent screening of hybridoma products against a "HB-X-GST" fusion construct. Has been used successfully for the detection of the hepatitis B X antigen by immunohistochemistry in formalin fixed, paraffin embedded material, also by western blotting against the immunizing and screening fusion proteins. Subsequently clone 3F6-G10 has been used as a capture reagent in a sensitive sandwich ELISA and bead based flow assay for the quantitative assessment of HbX antigen in human sera. Fine epitope mapping by phage library screening indicates that the epitope recognized bylies between amino acids 88 and 93 of the X antigen, a result subsequently confirmed by peptide ELISA.
Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
PBS, pH 7.2, 0.09% sodium azide, 0.1% BSA.
+4°C or -20°C, Avoid repeated freezing and thawing. Store undiluted.