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Anti-HEDLS / EDC4 Antibody (clone 2E2) IHC-plus™ LS-B6221

Note: This antibody replaces LS-C134051
Catalog Size Price
LS-B6221-50 50 µg (0.5 mg/ml) Unavailable

Related Products

11 HEDLS / EDC4 Antibodies

Most Popular HEDLS / EDC4 Antibodies

Anti-HEDLS / EDC4 Antibody (aa74-123) LS-C81984
Rabbit Polyclonal (IgG) to Human HEDLS / EDC4
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Western blot
Unconjugated
Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-HEDLS / EDC4 Antibody (clone 2E2) IHC-plus™ LS-B6221
Mouse Monoclonal [clone 2E2] (IgG2a,k) to Human HEDLS / EDC4
Human
IHC, IHC - Paraffin, ELISA
Unconjugated
Anti-EDC4 / Ge-1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6221 concentration 3 ug/ml.
Anti-HEDLS / EDC4 Antibody (aa1-50) LS-C287608
Rabbit Polyclonal (IgG) to Human HEDLS / EDC4
Human, Mouse
Western blot, Immunoprecipitation, Proximity Ligation Assay
Unconjugated
Anti-HEDLS / EDC4 Antibody LS-C665277
Rabbit Polyclonal (IgG) to Human HEDLS / EDC4
Human
IHC, Western blot, ELISA
Unconjugated
Anti-HEDLS / EDC4 Antibody LS-C671864
Rabbit Polyclonal (IgG) to Human HEDLS / EDC4
Human
Unconjugated
Immunohistochemistry image at a dilution of 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

100% Guaranteed
Mouse Monoclonal [clone 2E2] (IgG2a,k) to Human HEDLS / EDC4
Human
IHC, IHC - Paraffin, ELISA
Unconjugated

Details

Human HEDLS / EDC4
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a,k Monoclonal [2E2]
Unconjugated
Purified from ascites by Protein A
Unmodified

Applications

  • IHC
  • IHC - Paraffin (3 - 5 µg/ml)
  • ELISA

Specificity and Use

HEDLS / EDC4 antibody was raised against eDC4 (NP_055144, 1302 a.a. ~ 1402 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human EDC4
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein.

Packaging

PBS, pH 7.4
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
For research use only.

About HEDLS / EDC4

Q6P2E9 NM_014329 NP_055144.3

EDC4 Antibody, Autoantigen Ge-1 Antibody, Ge-1 Antibody, HEDL5 Antibody, RCD-8 Antibody, RCD8 Antibody, Autoantigen RCD-8 Antibody, Enhancer of mRNA decapping 4 Antibody, GE1 Antibody, HEDLS Antibody

In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).

Immunohistochemistry

Anti-EDC4 / Ge-1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6221 concentration 3 ug/ml.
Anti-EDC4 / Ge-1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6221 concentration 3 ug/ml.

Immunohistochemistry

Anti-EDC4 / Ge-1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6221 concentration 3 ug/ml.
Anti-EDC4 / Ge-1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6221 concentration 3 ug/ml.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged EDC4 is 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged EDC4 is 0.3 ng/ml as a capture antibody.

Requested From: 
Date Requested: 5/22/2018

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