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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1704-50 | 50 µl (3.44 mg/ml) | $460 |
IHC‑plus™ Monoclonal Mouse anti‑Human HEC1 / NDC80 Antibody (clone 9G3.23, aa56‑642, IHC, IF, WB) LS‑B1704
IHC‑plus™ Monoclonal Mouse anti‑Human HEC1 / NDC80 Antibody (clone 9G3.23, aa56‑642, IHC, IF, WB) LS‑B1704
Note: This antibody replaces LS-C20070
Antibody:
HEC1 / NDC80 Mouse anti-Human Monoclonal (aa56-642) (9G3.23) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
HEC1 / NDC80 Mouse anti-Human Monoclonal (aa56-642) (9G3.23) Antibody
Application:
IHC, IHC-P, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
NDC80 antibody LS-B1704 is an unconjugated mouse monoclonal antibody to human NDC80 (HEC1) (aa56-642). Validated for IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human HEC1 / NDC80
Synonyms
NDC80 | HEC1 | Kinetochore protein Hec1 | Kinetochore associated 2 | HEC | HsHec1 | HsNDC80 | KNTC2 | TID3
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Monoclonal
Clone
9G3.23
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Human HEC1 protein consisting of amino acids 56-642.
Epitope
aa56-642
Specificity
Human HEC1 protein consisting of amino acids 56-642.
Applications
- IHC
- IHC - Paraffin (1:50)
- Immunofluorescence
- Western blot (1:500 - 1:3000)
- Immunoprecipitation
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1704 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B1704 was determined to be 1:50.
Presentation
PBS
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About HEC1 / NDC80
Validation
Anti-NDC80 / HEC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-NDC80 / HEC1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ HEC1 / NDC80 Antibody (aa56-642, clone 9G3.23) for IHC, IF/Immunofluorescence, WB/Western, IP LS-B1704 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human
Applications:
ICC, Western blot, Flow Cytometry, Dot Blot
Species:
Human, Mouse, Rat
Applications:
IHC, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Reactivity:
Human, Mouse
Range:
Positive/Negative
Reactivity:
Mouse
Range:
0.39-25 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
