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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Heat Shock Protein 70 / HSPA1A Antibody (Tyr525) IHC‑plus™ LS‑B9063
Note: This antibody replaces LS-C100203
HSPA1A antibody LS-B9063 is an unconjugated rabbit polyclonal antibody to human HSPA1A (Heat Shock Protein 70). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B9063-200
200 µl
Unavailable

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Product Description

HSPA1A antibody LS-B9063 is an unconjugated rabbit polyclonal antibody to human HSPA1A (Heat Shock Protein 70). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
About Heat Shock Protein 70 / HSPA1A
In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins.

Specifications

Target
Human Heat Shock Protein 70 / HSPA1A
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E9068 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B9063. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Tyr525
Specificity
This HSPA1A/HSPA1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 503-532 amino acids from human HSPA1A/HSPA1B.
Presentation
PBS with 0.09% (w/v) sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry - Paraffin

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot of HSPA1A (arrow) using rabbit polyclonal HSPA1A Antibody (Y525). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPA1A gene (Lane 2) (Origene Technologies).
Western blot of HSPA1A (arrow) using rabbit polyclonal HSPA1A Antibody (Y525). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPA1A gene (Lane 2) (Origene Technologies).

Immunohistochemistry - Paraffin

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot of HSPA1A (arrow) using rabbit polyclonal HSPA1A Antibody (Y525). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPA1A gene (Lane 2) (Origene Technologies).
Western blot of HSPA1A (arrow) using rabbit polyclonal HSPA1A Antibody (Y525). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPA1A gene (Lane 2) (Origene Technologies).

Immunohistochemistry - Paraffin

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot of HSPA1A (arrow) using rabbit polyclonal HSPA1A Antibody (Y525). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPA1A gene (Lane 2) (Origene Technologies).
Western blot of HSPA1A (arrow) using rabbit polyclonal HSPA1A Antibody (Y525). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPA1A gene (Lane 2) (Origene Technologies).

Immunohistochemistry - Paraffin

Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western blot

Western blot of HSPA1A (arrow) using rabbit polyclonal HSPA1A Antibody (Y525). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPA1A gene (Lane 2) (Origene Technologies).
Western blot of HSPA1A (arrow) using rabbit polyclonal HSPA1A Antibody (Y525). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HSPA1A gene (Lane 2) (Origene Technologies).


Requested From: 
Date Requested: 6/22/2018

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