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Anti-HDAC9 Antibody (aa2-32) LS-C96661

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HDAC9 Antibodies

Anti-HDAC9 Antibody (aa503-533) LS-C96662
Rabbit Polyclonal to Human HDAC9
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HDAC9 Antibody (N-Terminus) IHC-plus™ LS-B10810
Rabbit Polyclonal to Human HDAC9
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-HDAC9 Antibody (Internal) IHC-plus™ LS-B10811
Rabbit Polyclonal to Human HDAC9
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human HDAC9
Human
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated

Details

Human HDAC9
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence
  • Western blot (1:1000)
  • Immunoprecipitation (1:100)

Specificity and Use

HDAC9 antibody was raised against kLH-conjugated synthetic peptide between amino acids 2-32 from N-terminal region of human HDAC9.
LS-E741 - Lyophilized - 100 µg - $145.00
aa2-32

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

Publications (1)

1
Mirk/dyrk1B decreases the nuclear accumulation of class II histone deacetylases during skeletal muscle differentiation. Deng X, Ewton DZ, Mercer SE, Friedman E. The Journal of biological chemistry. 2005 280:4894-905. [PubMed:15546868]

About HDAC9

Q9UKV0 NM_014707 NP_055522.1

HDAC9 Antibody, HD7 Antibody, HD9 Antibody, HDAC Antibody, HDAC7B Antibody, HDAC9B Antibody, HDAC9FL Antibody, KIAA0744 Antibody, HD7b Antibody, HDRP Antibody, Histone deacetylase 7B Antibody, Histone deacetylase 9 Antibody, MITR Antibody

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3.

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Immunofluorescence

Immunofluorescence
Immunofluorescence staining of MITR for a compartmentalization study in undifferentiated C2C12 myoblasts transfected with a MITR-expressing plasmid. MITR is detected by using the HDAC9 N-term antibody (top panel) or a FLAG antibody (bottom panel) detecting a FLAG epitope fused at the N-term end of the MITR construct. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.

Western blot

Western blot
Both anti-HDAC9 N-term and C-term antibody were tested by WB and IP-WB using HeLa and HeLa-HDAC9 transfected cells. Top figure shows both antibody specifically detect HDAC9 in HeLa-HDAC9 transfected cell but not HeLa alone.

Western blot

Western blot
Figure 1: Immunoblots for MITR / HDAC9 N-term antibody), Mirk, MyoD and tubulin proteins are shown for cytoplasmic (Cyt) and nuclear (N) extracts from undifferentiated C2C12 myoblasts. Before cell collection for fractionation, the cells are transfected with plasmids coding for Mirk (Wt), kinase-inactive Mirk (YF) or MITR. Data courtesy of laboratory of Dr. Eileen Friedman. Dept of Pathology, Upstate Medical University, State University of New York.

Immunoprecipitation

Immunoprecipitation
This figure shows that both antibody can immunoprecipitate (IP) HDAC9 from HeLa-HDAC9 transfected cells. (Data kindly provided by Dr. Zhigang Yuan, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL).
 

Requested From: United States
Date Requested: 12/4/2016

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