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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HDAC8 Antibody (clone 2F4) LS‑C139403
HDAC8 antibody LS-C139403 is an unconjugated mouse monoclonal antibody to human HDAC8. Validated for ELISA, IP and WB.
Catalog
Size
Price
LS-C139403-100
100 µg (1 mg/ml)
Unavailable

Popular HDAC8 Products

Antibody
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation
Anti-HDAC8 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B967 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Immunohistochemical analysis of Histone Deacetylase 8 (pS39) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot

Product Description

HDAC8 antibody LS-C139403 is an unconjugated mouse monoclonal antibody to human HDAC8. Validated for ELISA, IP and WB.
About HDAC8
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. Q9BY41 NM_018486 NP_060956.1

HDAC8 Antibody, CDLS5 Antibody, HD8 Antibody, HDACL1 Antibody, Histone deacetylase 8 Antibody, MRXS6 Antibody, RPD3 Antibody, CDA07 Antibody, Histone deacetylase-like 1 Antibody, WTS Antibody


Specifications

Target
Human HDAC8
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [2F4]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • ELISA
Immunogen
HDAC8 antibody was raised against hDAC8 (NP_060956, 1 a.a. ~ 101 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human HDAC8
Usage
Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4.
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western blot of HDAC8 expression in transfected 293T cell line by HDAC8 monoclonal antibody (M07), clone 2F4.
Western blot of HDAC8 expression in transfected 293T cell line by HDAC8 monoclonal antibody (M07), clone 2F4.

Immunoprecipitation

Immunoprecipitation of HDAC8 transfected lysate using anti-HDAC8 monoclonal antibody and Protein A Magnetic Bead.
Immunoprecipitation of HDAC8 transfected lysate using anti-HDAC8 monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HDAC8 is 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HDAC8 is 0.3 ng/ml as a capture antibody.

Western blot

Western blot of HDAC8 expression in transfected 293T cell line by HDAC8 monoclonal antibody (M07), clone 2F4.
Western blot of HDAC8 expression in transfected 293T cell line by HDAC8 monoclonal antibody (M07), clone 2F4.

Immunoprecipitation

Immunoprecipitation of HDAC8 transfected lysate using anti-HDAC8 monoclonal antibody and Protein A Magnetic Bead.
Immunoprecipitation of HDAC8 transfected lysate using anti-HDAC8 monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HDAC8 is 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HDAC8 is 0.3 ng/ml as a capture antibody.

Western blot

Western blot of HDAC8 expression in transfected 293T cell line by HDAC8 monoclonal antibody (M07), clone 2F4.
Western blot of HDAC8 expression in transfected 293T cell line by HDAC8 monoclonal antibody (M07), clone 2F4.

Immunoprecipitation

Immunoprecipitation of HDAC8 transfected lysate using anti-HDAC8 monoclonal antibody and Protein A Magnetic Bead.
Immunoprecipitation of HDAC8 transfected lysate using anti-HDAC8 monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HDAC8 is 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HDAC8 is 0.3 ng/ml as a capture antibody.

Western blot

Western blot of HDAC8 expression in transfected 293T cell line by HDAC8 monoclonal antibody (M07), clone 2F4.
Western blot of HDAC8 expression in transfected 293T cell line by HDAC8 monoclonal antibody (M07), clone 2F4.

Immunoprecipitation

Immunoprecipitation of HDAC8 transfected lysate using anti-HDAC8 monoclonal antibody and Protein A Magnetic Bead.
Immunoprecipitation of HDAC8 transfected lysate using anti-HDAC8 monoclonal antibody and Protein A Magnetic Bead.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged HDAC8 is 0.3 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged HDAC8 is 0.3 ng/ml as a capture antibody.


Requested From: 
Date Requested: 6/20/2018

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