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Anti-HDAC8 Antibody (C-Terminus) IHC-plus™ LS-B967

Note: This antibody replaces LS-C29796

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HDAC8 Antibodies

Anti-HDAC8 Antibody (C-Terminus) IHC-plus™ LS-B967
Rabbit Polyclonal to Human HDAC8
Human, Monkey, Mouse, Rat, Bovine, Horse, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HDAC8 Antibody (clone 2F4) LS-C139403
Mouse Monoclonal [clone 2F4] (IgG2a,k) to Human HDAC8
Human
Western blot, Immunoprecipitation, ELISA
Unconjugated
Western blot Image
Anti-HDAC8 Antibody (N-Terminus, FITC) LS-C244611
Rabbit Polyclonal (IgG) to Human HDAC8
Human
IHC, Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human HDAC8
Human, Monkey, Mouse, Rat, Bovine, Horse, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human HDAC8
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Horse, Rabbit (tested or 100% immunogen sequence identity)
Guinea pig, Chicken, Xenopus, Zebrafish (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

HDAC8 antibody was raised against synthetic peptide from C-Terminus of human HDAC8 (Q9BY41, NP_060956). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Rabbit, Horse (100%); Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Stickleback, Zebrafish (92%).
LS-E12284 - Lyophilized - 100 µg - $145.00
C-Terminus
Human HDAC8
Immunohistochemistry: LS-B967 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B967 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About HDAC8

Q9BY41 NM_018486 NP_060956.1

HDAC8 Antibody, CDLS5 Antibody, HD8 Antibody, HDACL1 Antibody, Histone deacetylase 8 Antibody, MRXS6 Antibody, RPD3 Antibody, CDA07 Antibody, Histone deacetylase-like 1 Antibody, WTS Antibody

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin.

Immunohistochemistry

Immunohistochemistry
Anti-HDAC8 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B967 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-HDAC8 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B967 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
HDAC8 antibody LS-B967 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Jurkat Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 12/9/2016

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